Evidence map›Paper›PMID 34565457›Full record

ArticleBMC research notes2021

Improved library preparation protocols for amplicon sequencing-based noninvasive fetal genotyping for RHD-positive D antigen-negative alleles.

Asuka Hori, Hiroko Ogata-Kawata, Aiko Sasaki, Ken Takahashi, Kosuke Taniguchi, Ohsuke Migita, Akihiro Kawashima, Aikou Okamoto, Akihiko Sekizawa, Haruhiko Sago and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC research notes, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Asuka HoriDepartment of Maternal-Fetal Biology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo, 157-8535, Japan.
Hiroko Ogata-KawataDepartment of Maternal-Fetal Biology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo, 157-8535, Japan.
Aiko SasakiCenter for Maternal-Fetal, Neonatal, and Reproductive Medicine, National Center for Child Health and Development, Tokyo, Japan.
Ken TakahashiDepartment of Obstetrics and Gynecology, The Jikei University School of Medicine, Tokyo, Japan.
Kosuke TaniguchiDepartment of Maternal-Fetal Biology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo, 157-8535, Japan.
Ohsuke MigitaDepartment of Maternal-Fetal Biology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo, 157-8535, Japan.
Akihiro KawashimaDepartment of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan.
Aikou OkamotoDepartment of Obstetrics and Gynecology, The Jikei University School of Medicine, Tokyo, Japan.
Akihiko SekizawaDepartment of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan.
Haruhiko SagoCenter for Maternal-Fetal, Neonatal, and Reproductive Medicine, National Center for Child Health and Development, Tokyo, Japan.
Fumio TakadaDepartment of Medical Genetics and Genomics, Kitasato University Graduate School of Medical Sciences, Kanagawa, Japan.
Kazuhiko Nakabayashi *Laboratory of Developmental Genomics, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo, 157-8535, Japan. nakabaya-k@ncchd.go.jp.ORCID http://orcid.org/0000-0003-2927-0963
Kenichiro Hata *Department of Maternal-Fetal Biology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo, 157-8535, Japan. hata-k@ncchd.go.jp.

Funding

National Center for Child Health and Development of Japan 2020B-9
6 · The paper itself

Abstract

objectiveWe aimed to simplify our fetal RHD genotyping protocol by changing the method to attach Illumina's sequencing adaptors to PCR products from the ligation-based method to a PCR-based method, and to improve its reliability and robustness by introducing unique molecular indexes, which allow us to count the numbers of DNA fragments used as PCR templates and to minimize the effects of PCR and sequencing errors.

resultsBoth of the newly established protocols reduced time and cost compared with our conventional protocol. Removal of PCR duplicates using UMIs reduced the frequencies of erroneously mapped sequences reads likely generated by PCR and sequencing errors. The modified protocols will help us facilitate implementing fetal RHD genotyping for East Asian populations into clinical practice.

Indexed as

Prenatal DiagnosisRh-Hr Blood-Group SystemAllelesFemaleGenotypeHumansPregnancyPrenatal CareReproducibility of ResultsRh-Hr Blood-Group SystemAmplicon sequencingCell-free DNA (cfDNA)Non-invasive prenatal testing (NIPT)RHDUnique molecular identifier (UMI)

Identifiers

PMID34565457
PMCPMC8474863

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.