Evidence map›Paper›PMID 34565303›Full record

ArticleBioengineered2021

MicroRNA-140-5p represses chondrocyte pyroptosis and relieves cartilage injury in osteoarthritis by inhibiting cathepsin B/Nod-like receptor protein 3.

Lei Zhang, Jianjun Qiu, Jixiang Shi, Shaoyang Liu, Hanlin Zou

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioengineered, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
2.8field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 42 citations in OpenAlex.

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  17. Ferroptosis in Osteoarthritis: Current Understanding.Journal of inflammation research · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Lei ZhangDepartment of Orthopedics, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Jianjun QiuDepartment of Orthopedics, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Jixiang ShiDepartment of Orthopedics, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Shaoyang LiuDepartment of Orthopedics, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Hanlin ZouDepartment of Orthopedics, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.ORCID 0000-0002-7456-9764
Shanghai University of Traditional Chinese Medicine · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Osteoarthritis (OA) is a degenerative joint disease. Dysregulated microRNA (miRNA) expressions are implicated in OA progression. Consequently, the current study set out to investigate the mechanism of miR-140-5p in OA cartilage injury. Firstly, the murine and cell models of OA were established, and cartilage tissues of OA mice were observed using hematoxylin and eosin staining and safranin O staining. Chondrocyte pyroptosis was further assessed using immunohistochemical and Calcein-AM/PI staining. The levels of gasdermin-D (GSDMD)-N, cleaved caspase-1, interleukin (IL)-1β, and IL-18 in cartilage tissues and cells were determined using Western blot and enzyme-linked immunosorbent assay kits. The targeting relationship between miR-140-5p and cathepsin B (CTSB) was verified using a dual-luciferase assay. Moreover, the binding of CTSB and Nod-like receptor protein 3 (NLRP3) was detected using co-immunoprecipitation assay. Lastly, the effects of NLRP3 activation and CTSB overexpression on chondrocyte pyroptosis were documented. It was found that OA induction aggravated cartilage tissue injury and enhanced chondrocyte pyroptosis. miR-140-5p was poorly-expressed in OA models, and miR-140-5p over-expression alleviated chondrocyte pyroptosis, as evidenced by decreased GSDMD-N, cleaved caspase-1, IL-1β, and IL-18 levels. miR-140-5p targeted the CTSB gene, whereas CTSB further bound to NLRP3 and activated the NLRP3 inflammasome. Additionally, CTSB over-expression or NLRP3 activation reversed the inhibitory effect of miR-140-5p on chondrocyte pyroptosis. Collectively, our findings revealed that miR-140-5p repressed chondrocyte pyroptosis and alleviated OA cartilage injury

Indexed as

AnimalsBase SequenceCartilage, ArticularCathepsin BChondrocytesDisease Models, AnimalLipopolysaccharidesMaleMiceMice, Inbred BALB CMicroRNAsModels, BiologicalNLR Family, Pyrin Domain-Containing 3 ProteinOsteoarthritisProtein BindingPyroptosisCathepsin BLipopolysaccharidesMicroRNAsMIRN140 microRNA, mouseNLR Family, Pyrin Domain-Containing 3 ProteinCartilage injuryco-immunoprecipitationctsbmiR-140-5pnlrp3Osteoarthritispyroptosis

Identifiers

PMID34565303
PMCPMC8810115
OpenAlexW3203771579

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.