Evidence map›Paper›PMID 34562927›Full record

ArticleBiosensors2021

A Co-Culture-Based Multiparametric Imaging Technique to Dissect Local H

Melike Secilmis, Hamza Yusuf Altun, Johannes Pilic, Yusuf Ceyhun Erdogan, Zeynep Cokluk, Busra Nur Ata, Gulsah Sevimli, Asal Ghaffari Zaki, Esra Nur Yigit, Gürkan Öztürk and 2 more

Abstract read
In one paragraph

Article in Biosensors, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Proteome-wide tagging with an HProceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Melike SecilmisMolecular Biology, Genetics and Bioengineering Program, Sabanci University, 34956 Istanbul, Turkey.ORCID 0000-0002-0749-1821
Hamza Yusuf AltunMolecular Biology, Genetics and Bioengineering Program, Sabanci University, 34956 Istanbul, Turkey.ORCID 0000-0003-4207-6946
Johannes PilicMolecular Biology and Biochemistry, Gottfried Schatz Research Center, Medical University of Graz, 8036 Graz, Austria.ORCID 0000-0001-8452-0879
Yusuf Ceyhun ErdoganMolecular Biology, Genetics and Bioengineering Program, Sabanci University, 34956 Istanbul, Turkey.ORCID 0000-0001-8878-6930
Zeynep CoklukMolecular Biology, Genetics and Bioengineering Program, Sabanci University, 34956 Istanbul, Turkey.
Busra Nur AtaMolecular Biology, Genetics and Bioengineering Program, Sabanci University, 34956 Istanbul, Turkey.
Gulsah SevimliMolecular Biology, Genetics and Bioengineering Program, Sabanci University, 34956 Istanbul, Turkey.ORCID 0000-0003-0485-740X
Asal Ghaffari ZakiMolecular Biology, Genetics and Bioengineering Program, Sabanci University, 34956 Istanbul, Turkey.ORCID 0000-0001-7446-8747
Esra Nur YigitResearch Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University, 34810 Istanbul, Turkey.ORCID 0000-0001-9037-5217
Gürkan ÖztürkResearch Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University, 34810 Istanbul, Turkey.
Roland MalliMolecular Biology and Biochemistry, Gottfried Schatz Research Center, Medical University of Graz, 8036 Graz, Austria.ORCID 0000-0001-6327-8729
Emrah ErogluMolecular Biology, Genetics and Bioengineering Program, Sabanci University, 34956 Istanbul, Turkey.ORCID 0000-0002-9373-0808

Funding

Austrian Science Fund FWF I 3716Austrian Science Fund FWF J 4113
6 · The paper itself

Abstract

Multispectral live-cell imaging is an informative approach that permits detecting biological processes simultaneously in the spatial and temporal domain by exploiting spectrally distinct biosensors. However, the combination of fluorescent biosensors with distinct spectral properties such as different sensitivities, and dynamic ranges can undermine accurate co-imaging of the same analyte in different subcellular locales. We advanced a single-color multiparametric imaging method, which allows simultaneous detection of hydrogen peroxide (H

Indexed as

Biosensing TechniquesCoculture TechniquesEndothelial CellsHydrogen PeroxideMitochondriaHydrogen Peroxideco-imagingendothelial cell linehydrogen peroxideHyPer7mitochondriamultiparametric imagingmultiplex live-cell imagingstable cell linestargeted biosensors

Identifiers

PMID34562927
PMCPMC8466187

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.