Expression, Purification, and Structure Determination of Human PTCH1-HH-N Complexes.

Xiaofeng Qi, Philip Schmiege, Leticia Esparza, Xiaochun Li

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.5field-weighted citation impact, top 36% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

Biology · 2024
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Xiaofeng QiDepartment of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX, USA. Xiaofeng.Qi@UTSouthwestern.edu.

Philip SchmiegeDepartment of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX, USA.

Leticia EsparzaDepartment of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX, USA.

Xiaochun LiDepartment of Molecular Genetics and Biophysics, University of Texas Southwestern Medical Center, Dallas, TX, USA. Xiaochun.Li@UTSouthwestern.edu.

The University of Texas Southwestern Medical Center · US

Funding

Molecular Biophysics Training ProgramT32GM131963 · NIGMS · UT SOUTHWESTERN MEDICAL CENTER · PI Luke W Rice · 2019 to 2026

$1.5M

Structural and Functional investigations of Hedgehog Signaling TransductionR01GM135343 · NIGMS · UT SOUTHWESTERN MEDICAL CENTER · PI LI, XIAOCHUN · 2019 to 2022

$1.2M

NIGMS NIH HHS R01 GM135343NIGMS NIH HHS T32 GM131963

6 · The paper itself

Abstract

Patched-1 (PTCH1), a tumor suppressor, serves as the receptor of Hedgehog (HH) ligand and negatively regulates the HH signaling pathway. Mutations of PTCH1 are implicated in many human cancers. Structural investigation revealed the mechanism of PTCH1-mediated HH signal regulation, further facilitating the therapeutic development of cancers. Here, we describe the expression and purification of a nearly full-length functional PTCH1 variant, PTCH1*. With purified PTCH1* protein, two forms of PTCH1*-Sonic Hedgehog (SHH) complexes were assembled, and their structures subsequently determined by cryo-electron microscope (cryo-EM).

Indexed as

Signal TransductionCryoelectron MicroscopyGenes, Tumor SuppressorHedgehog ProteinsHumansPatched-1 ReceptorPatched ReceptorsProtein BindingHedgehog ProteinsPatched-1 ReceptorPatched ReceptorsPTCH1 protein, humanComplex assemblyCryo-EMHedgehogPatched-1Protein purification

Identifiers

PMID34562247

DOI10.1038/nmeth.4193

OpenAlexW3199740964

What OpenQuestion holds

Textmetadata

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.