Evidence map›Paper›PMID 34562246›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Analysis of Dispatched Protein Processing and Sonic Hedgehog Ligand Release.

Elizabeth Cleverdon, Daniel P Stewart, Stacey K Ogden

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 83% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 0 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Elizabeth CleverdonDepartment of Cell and Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN, USA.
Daniel P StewartDepartment of Cell and Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN, USA.
Stacey K OgdenDepartment of Cell and Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN, USA. Stacey.Ogden@stjude.org.
St. Jude Children's Research Hospital · US

Funding

Mechanisms of Sonic Hedgehog Signal TransductionR35GM122546 · NIGMS · ST. JUDE CHILDREN'S RESEARCH HOSPITAL · PI Stacey Kathryn Ogden · 2017 to 2026
$4.8M
NIGMS NIH HHS R35 GM122546
6 · The paper itself

Abstract

The 12-pass transmembrane protein Dispatched (DISP) is essential for Sonic Hedgehog (SHH) release from ligand-producing cells and is indispensable for establishment of the SHH morphogen gradient during tissue patterning. Regulatory events controlling DISP release of SHH are not yet fully characterized. We recently demonstrated that DISP is cleaved by FURIN proprotein convertase at a conserved site in its first extracellular loop. Mutation of the cleavage site attenuates DISP-mediated SHH release, which indicates that Furin cleavage is a positive step toward DISP protein maturation. In this chapter, we present a ligand release/retention protocol that allows for the analysis of DISP cleavage, DISP-mediated release of SHH ligand from producing cells, and secretion-dependent signal induction in target cells.

Indexed as

Signal TransductionFurinHedgehog ProteinsLigandsTrans-ActivatorsFurinHedgehog ProteinsLigandsTrans-ActivatorsDispatchedLigand release/retentionMorphogen releaseReporter assaySonic Hedgehog

Identifiers

PMID34562246
PMCPMC8864978
OpenAlexW3199586192

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.