Evidence map›Paper›PMID 34558119›Full record

ReviewMass spectrometry reviews2023

Recent advances in mass spectrometry analysis of neuropeptides.

Ashley Phetsanthad, Nhu Q Vu, Qing Yu, Amanda R Buchberger, Zhengwei Chen, Caitlin Keller, Lingjun Li

Abstract readReview
In one paragraph

Review in Mass spectrometry reviews, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. Article
  8. Review
  9. Review
  10. Article
  11. Article
  12. Neuropeptidomics of the American LobsterJournal of proteome research · 2024
    Article
  13. Article
  14. Article
  15. Origins, Technological Advancement, and Applications of Peptidomics.Methods in molecular biology (Clifton, N.J.) · 2024
    Article
  16. Peptidomics.Nature reviews. Methods primers · 2023
    Article
  17. Article
  18. Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Ashley PhetsanthadDepartment of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Nhu Q VuDepartment of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Qing YuSchool of Pharmacy, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Amanda R BuchbergerDepartment of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Zhengwei ChenDepartment of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Caitlin KellerDepartment of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Lingjun LiDepartment of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.

Funding

BIOTECHNOLOGY TRAINING PROGRAMT32GM008349 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI FOX, BRIAN G · 1989 to 2019
$22.5M
Training Program in Translational Cardiovascular Science (TPTCS)T32HL007936 · NHLBI · UNIVERSITY OF WISCONSIN-MADISON · PI Lee Lochbaum Eckhardt, Gail A Robertson · 2001 to 2026
$11.6M
Chemistry-Biology Interface Training ProgramT32GM008505 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI BLACKWELL, HELEN E. · 1993 to 2023
$9.8M
STATE DEPENDENT COORDINATION OF RHYTHMIC NEURAL CIRCUITSR01NS029436 · NINDS · UNIVERSITY OF PENNSYLVANIA · PI MICHAEL P NUSBAUM · 1991 to 2026
$7.4M
Mass Spectrometric Studies of Neuropeptides in FeedingR01DK071801 · NIDDK · UNIVERSITY OF WISCONSIN-MADISON · PI LINGJUN LI · 2006 to 2026
$6.7M
In Situ Protein Characterization and Visualization in the Brains of Mouse Models for AutismR56MH110215 · NIMH · UNIVERSITY OF WISCONSIN-MADISON · PI IKONOMIDOU, HRISSANTHI, LI, LINGJUN · 2017 to 2018
$752k
Multifaceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus during HypoxiaF31GM119365 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI BUCHBERGER JONES, AMANDA RAE · 2016 to 2017
$62k
NHLBI NIH HHS T32 HL007936NIDDK NIH HHS R01 DK071801NIGMS NIH HHS F31 GM119365NIGMS NIH HHS T32 GM008349NIGMS NIH HHS T32 GM008505NIMH NIH HHS R56 MH110215NINDS NIH HHS R01 NS029436
6 · The paper itself

Abstract

Due to their involvement in numerous biochemical pathways, neuropeptides have been the focus of many recent research studies. Unfortunately, classic analytical methods, such as western blots and enzyme-linked immunosorbent assays, are extremely limited in terms of global investigations, leading researchers to search for more advanced techniques capable of probing the entire neuropeptidome of an organism. With recent technological advances, mass spectrometry (MS) has provided methodology to gain global knowledge of a neuropeptidome on a spatial, temporal, and quantitative level. This review will cover key considerations for the analysis of neuropeptides by MS, including sample preparation strategies, instrumental advances for identification, structural characterization, and imaging; insightful functional studies; and newly developed absolute and relative quantitation strategies. While many discoveries have been made with MS, the methodology is still in its infancy. Many of the current challenges and areas that need development will also be highlighted in this review.

Indexed as

NeuropeptidesMass SpectrometryNeuropeptidesimagingmass spectrometrymicrodialysisneuropeptidesposttranslational modificationsquantitation

Identifiers

PMID34558119
PMCPMC9067165

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.