Evidence map›Paper›PMID 34558051›Full record

ArticleEnvironmental science and pollution research international2022

Embryotoxic effects of tribromophenol on early post-implantation development of mouse embryos in vitro.

Zhihua Zhao, Dicong Zhu, Yujie Liu, Qing Zhou, Jingfan Qiu, Cheng Xu, Yuanlin He, Wentao Zeng, Yang Yang

Abstract read
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In one paragraph

Article in Environmental science and pollution research international, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Artificial Uterus and Artificial Embryos: Unsolved Tasks.Reproductive sciences (Thousand Oaks, Calif.) · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Zhihua Zhao *State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, 211166, China.
Dicong Zhu *State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, 211166, China.
Yujie LiuState Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, China.
Qing ZhouState Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, China.
Jingfan QiuKey Laboratory of Pathogen Biology of Jiangsu Province, Department of Pathogen Biology, Nanjing Medical University, Nanjing, 211166, China.
Cheng XuState Key Laboratory of Reproductive Medicine, Institute of Toxicology, Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, 211166, China.
Yuanlin HeState Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, 211166, China.
Wentao ZengAnimal Core Facility, Nanjing Medical University, Nanjing, 211166, China.
Yang YangState Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, 211166, China. yangyang11@njmu.edu.cn.ORCID http://orcid.org/0000-0002-9887-7921
Nanjing Medical University · CNState Key Laboratory of Pollution Control and Resource Reuse · CNState Key Laboratory of Reproductive Medicine

Funding

China Postdoctoral Science Foundation 2019M651894China Postdoctoral Science Foundation 2019M651895High Level Youth Talents Cultivation Project of Nanjing Medical University 2019NJMURC011Jiangsu Postdoctoral Research Foundation 2019Z376National Natural Science Foundation of China 31801205National Natural Science Foundation of China 32070804Natural Science Foundation of Jiangsu Province BK20180677Natural Science Foundation of Jiangsu Province BK20200675Science and Technology Development Foundation of Nanjing Medical University NMUB2019034Six Talent Peaks Project in Jiangsu Province WSW-035
6 · The paper itself

Abstract

2,4,6-Tribromophenol (TBP, CAS No. 118-79-6), the most widely produced brominated phenol, is frequently detected in environmental components. The detection of TBP in human bodies has earned great concerns about its adverse effects on human beings, especially for early embryonic development. Here, we optimized the mouse embryo in vitro culture (IVC) system for early post-implantation embryos and employed it to determine the embryotoxicity of TBP. With this new research model, we revealed the dose-dependent toxic effects of TBP on mouse embryos from peri-implantation to egg cylinder stages. Furthermore, TBP exposure inhibited the differentiation and survival of epiblast (EPI) cells and extraembryonic endoderm (ExEn) cells, while those of extraembryonic ectoderm (ExEc) cells were not influenced. These results implied that TBP might inhibit embryonic development by influencing the generation of three primary germ layers and fetal membranes (the amnion, chorionic disk, umbilical cord, and yolk sac). In summary, we showed a proof of concept for applying mouse embryo IVC system as a novel research model for studying mammalian embryonic toxicology of environmental pollutants. This study also demonstrated the toxicity of TBP on early embryonic development of mammals.

Indexed as

Embryo, MammalianEmbryonic DevelopmentAnimalsCell DifferentiationFemaleMicePregnancy2,4,6-TribromophenolEmbryotoxicityIn vitro cultureMouse embryosPost-implantationThree-dimensional (3D) Matrigel drops

Identifiers

PMID34558051
OpenAlexW3201495212

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.