ArticleNature structural & molecular biology2021
Structural basis of the complete poxvirus transcription initiation process.
Article in Nature structural & molecular biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- Cooperative clamp-mediated promoter recognition by poxviral RNA polymerase and its TBP/TFIIB-like partner.Nature communications · 2026Article
- Indirect ELISA Based on ASFV Polymerase Three Subunits for Serological Monitoring of African Swine Fever Antibodies.Veterinary sciences · 2026Article
- Host transfer RNA guides assembly of viral RNA polymerase.Nature structural & molecular biology · 2025Article
- tRNA as an assembly chaperone for a macromolecular transcription-processing complex.Nature structural & molecular biology · 2025Article
- Structural basis of RNA polymerase complexes in African swine fever virus.Nature communications · 2025Article
- Methods to Study Poxvirus Structures by Cryo-EM Imaging Modalities.Methods in molecular biology (Clifton, N.J.) · 2025Article
- An Evolutionary Framework Exploiting Virologs and Their Host Origins to Inform Poxvirus Protein Functions.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Transcription regulation of African swine fever virus: dual role of M1249L.Nature communications · 2024Article
- Enzymatic characterization and dominant sites of foot-and-mouth disease virus 2C protein.Heliyon · 2024Article
- Structure of the recombinant RNA polymerase from African Swine Fever Virus.Nature communications · 2024Article
- Transcription termination and readthrough in African swine fever virus.Frontiers in immunology · 2024Article
- Genistein is effective in inhibiting Orf virus infectionFrontiers in microbiology · 2024Article
- Poxvirus under the eyes of electron microscope.Applied microscopy · 2022Review
- A generic protocol for the affinity-purification of native macromolecular complexes from poxvirus-infected cells.STAR protocols · 2022Article
- African Swine Fever Virus and Host Response: Transcriptome Profiling of the Georgia 2007/1 Strain and Porcine Macrophages.Journal of virology · 2022Article
- Vaccinia virus D10 has broad decapping activity that is regulated by mRNA splicing.PLoS pathogens · 2022Article
- Evolutionary dissection of monkeypox virus: Positive Darwinian selection drives the adaptation of virus-host interaction proteins.Frontiers in cellular and infection microbiology · 2022Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Poxviruses express their genes in the cytoplasm of infected cells using a virus-encoded multi-subunit polymerase (vRNAP) and unique transcription factors. We present cryo-EM structures that uncover the complete transcription initiation phase of the poxvirus vaccinia. In the pre-initiation complex, the heterodimeric early transcription factor VETFs/l adopts an arc-like shape spanning the polymerase cleft and anchoring upstream and downstream promoter elements. VETFI emerges as a TBP-like protein that inserts asymmetrically into the DNA major groove, triggers DNA melting, ensures promoter recognition and enforces transcription directionality. The helicase VETFs fosters promoter melting and the phospho-peptide domain (PPD) of vRNAP subunit Rpo30 enables transcription initiation. An unprecedented upstream promoter scrunching mechanism assisted by the helicase NPH-I probably fosters promoter escape and transition into elongation. Our structures shed light on unique mechanisms of poxviral gene expression and aid the understanding of thus far unexplained universal principles in transcription.
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