ArticleBiomaterials research2021
Enhanced osteogenic differentiation of alendronate-conjugated nanodiamonds for potential osteoporosis treatment.
Article in Biomaterials research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Enhancing Osteogenesis in Osteoporosis via Electromagnetized Gold Nanoparticles.Biomaterials research · 2025Article
- Nanodiamond-mediated delivery of microRNA-7 for the neuroprotection of dopaminergic neurons.Frontiers in bioengineering and biotechnology · 2024Article
- Emerging nano-scale delivery systems for the treatment of osteoporosis.Biomaterials research · 2023Review
- A systematic study on the use of multifunctional nanodiamonds for neuritogenesis and super-resolution imaging.Biomaterials research · 2023Article
- Nitric Oxide-Releasing Bioinspired Scaffold for Exquisite Regeneration of Osteoporotic Bone via Regulation of Homeostasis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- State of the Art in Carbon Nanomaterials for Photoacoustic Imaging.Biomedicines · 2022Review
- Arctiin elevates osteogenic differentiation of MC3T3-E1 cells by modulating cyclin D1.Bioengineered · 2022Article
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6 authors.
Funding
Abstract
backgroundAlendronate (Alen) is promising material used for bone-targeted drug delivery due to its high bone affinity and therapeutic effects on bone diseases. In addition, Alen can enhance the osteogenic differentiation of osteoblastic cell. Recently, nanodiamonds (NDs) with hardness, non-toxicity, and excellent biocompatibility are employed as promising materials for carrier systems and osteogenic differentiation. Therefore, we prepared Alen-conjugated NDs (Alen-NDs) and evaluated their osteogenic differentiation performances.
methodsAlen-NDs were synthesized using DMTMM as a coupling reagent. Morphological change of Mouse calvaria-derived preosteoblast (MC3T3-E1) treated with Alen-NDs was observed using the confocal microscope. The osteogenic differentiation was confirmed by cell proliferation, alkaline phosphatase (ALP), calcium deposition, and real-time polymerase chain reaction assay.
resultsAlen-NDs were prepared to evaluate their effect on the proliferation and differentiation of osteoblastic MC3T3-E1 cells. The Alen-NDs had a size of about 100 nm, and no cytotoxicity at less than 100 μg/mL of concentration. The treatment of NDs and Alen-NDs reduced the proliferation rate of MC3T3-E1 cells without cell death. Confocal microscopy images confirmed that the treatment of NDs and Alen-NDs changed the cellular morphology from a fibroblastic shape to a cuboidal shape. Flow cytometry, alkaline phosphatase (ALP) activity, calcium deposition, and real-time polymerase chain reaction (RT-PCR) confirmed the higher differentiation of MC3T3-E1 cells treated by Alen-NDs, compared to the groups treated by osteogenic medium and NDs. The higher concentration of Alen-ND treated in MC3T3-E1 resulted in a higher differentiation level.
conclusionsAlen-NDs can be used as potential therapeutic agents for osteoporosis treatment by inducing osteogenic differentiation.
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