Evidence map›Paper›PMID 34556115›Full record

ArticleBMC complementary medicine and therapies2021

In vitro antioxidant activity of crude extracts of Harpagophytum zeyheri and their anti-inflammatory and cytotoxicity activity compared with diclofenac.

Sibonokuhle F Ncube, Lyndy J McGaw, Emmanuel Mfotie Njoya, Hilton G T Ndagurwa, Peter J Mundy, Samson Sibanda

Open access · goldAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC complementary medicine and therapies, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.3field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Article
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  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 3 countries.

Sibonokuhle F NcubeDepartment of Forest Resources and Wildlife Management, Faculty of Applied Science, National University of Science and Technology, P. O. Box AC 939, Ascot, Bulawayo, Zimbabwe. sibonokuhle.ncube@nust.ac.zw.
Lyndy J McGawPhytomedicine Programme, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, 0110, South Africa.
Emmanuel Mfotie NjoyaPhytomedicine Programme, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, 0110, South Africa.
Hilton G T NdagurwaDepartment of Forest Resources and Wildlife Management, Faculty of Applied Science, National University of Science and Technology, P. O. Box AC 939, Ascot, Bulawayo, Zimbabwe.
Peter J MundyDepartment of Forest Resources and Wildlife Management, Faculty of Applied Science, National University of Science and Technology, P. O. Box AC 939, Ascot, Bulawayo, Zimbabwe.
Samson SibandaDepartment of Applied Chemistry, Faculty of Applied Science, National University of Science and Technology, P. O. Box AC 939, Ascot, Bulawayo, Zimbabwe.
National University of Science and Technology · ZWUniversity of Pretoria · ZA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThis study evaluated the in vitro antioxidant activity and comparison of anti-inflammatory and cytotoxic activity of Harpagopytum zeyheri with diclofenac.

methodsIn vitro assays were conducted using water, ethanol, and ethyl acetate extracts of H.zeyheri. The antioxidant activity was evaluated using the 2,2'-diphenyl-1-picrylhydrazy (DPPH) and 2,2'- azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays. The anti-inflammatory activity was determined by measuring the inhibition of nitric oxide (NO) on lipopolysaccharide (LPS)-induced RAW 264.7 mouse macrophages as well as cytokine (TNF-α and IL-10) expression on LPS-induced U937 human macrophages. For cytotoxicity, cell viability was determined using the 3-(4, 5-dimethylthiazol- 2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay.

resultsThe ethyl acetate extract had the lowest IC

conclusionsThese findings show that H. zeyheri has significant antioxidant activity. Additionally, similarities exist in the inflammatory activity of H. zeyheri to diclofenac at some concentrations as well as low toxicity in comparison to diclofenac.

Indexed as

HarpagophytumAnimalsAnti-Inflammatory Agents, Non-SteroidalAntioxidantsCytotoxinsDiclofenacHumansIn Vitro TechniquesMiceNitric OxidePlant ExtractsRAW 264.7 CellsU937 CellsZimbabweAnti-Inflammatory Agents, Non-SteroidalAntioxidantsCytotoxinsDiclofenacNitric OxidePlant ExtractsAntioxidantCytokinesCytotoxicityHarpagophytum zeyheriInflammationNitric oxide

Identifiers

PMID34556115
PMCPMC8461911
OpenAlexW3134493666

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.