ArticlePlant biotechnology journal2022
CRISPR-Cas9 mediated OsMIR168a knockout reveals its pleiotropy in rice.
Article in Plant biotechnology journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 76 citations in OpenAlex.
- Enhanced exonuclease-Cas9 systems promote multiple nucleotide deletions with higher efficiency and broader targeting scope in plants.Journal of integrative plant biology · 2026Article
- A rice ceRNA module suppresses Rhizoctonia solani-induced cross-kingdom RNAi to reduce fungal pathogenicity.Nature communications · 2026Article
- Exploring the CeRNA landscape in plants: advances, methods, and challenges.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Review
- Wheat miR408 and miR159 Weaken the Virulence ofPlants (Basel, Switzerland) · 2026Article
- Engineering non-coding DNA Elements in Rice: an Elegant Approach To fine-tune Agronomical Advantageous Traits.Rice (New York, N.Y.) · 2025Review
- MicroRNAs-mediated heat stress regulations in plants: Mechanisms and targets.The plant genome · 2025Review
- Natural variation of the MIR168a promoter influences its activity and miR168a abundance in rice.Rice (New York, N.Y.) · 2025Article
- Exploring the genetic basis of heterosis in eucalypt growth based on transcriptome analysis.BMC genomics · 2025Article
- Research Progress on Rice-Blast-Resistance-Related Genes.Plants (Basel, Switzerland) · 2025Review
- Identification and Characterization of Copper-Responsive miRNAs and Their Target Genes in Jerusalem Artichoke.Plants (Basel, Switzerland) · 2025Article
- CRISPR/Cas9: a sustainable technology to enhance climate resilience in major Staple Crops.Frontiers in genome editing · 2025Review
- PAM-relaxed and temperature-tolerant CRISPR-Mb3Cas12a single transcript unit systems for efficient singular and multiplexed genome editing in rice, maize, and tomato.Plant biotechnology journal · 2025Article
- Innovative opportunities for gene editing technology in crop breeding: from the perspective of literature analysis.Frontiers in plant science · 2025Article
- Review
- An efficient CRISPR-Cas12a-mediated MicroRNA knockout strategy in plants.Plant biotechnology journal · 2025Article
- Identification of multiple miRNA-encoded peptide reveals OsmiPEP162a putatively stabilizes OsMIR162 in rice.Plant cell reports · 2024Article
- Versatile plant genome engineering using anti-CRISPR-Cas12a systems.Science China. Life sciences · 2024Article
- Article
- Relationship between Protein, MicroRNA Expression in Extracellular Vesicles and Rice Seed Vigor.International journal of molecular sciences · 2024Article
- Expanding plant genome editing scope and profiles with CRISPR-FrCas9 systems targeting palindromic TA sites.Plant biotechnology journal · 2024Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNA168 (MIR168) is a key miRNA that targets the main RNA-induced silencing complex component Argonaute 1 (AGO1) to regulate plant growth and environmental stress responses. However, the regulatory functions of MIR168 need to be further elucidated in rice. In this paper, we generated clean OsMIR168a deletion mutants by CRISPR-Cas9 strategy. We then phenotypically and molecularly characterized these mutants. The rice OsMIR168a mutants grew rapidly at the seedling stage, produced more tillers and matured early. Compared to the wild-type plants, the mutants were shorter at maturity and produced smaller spikelets and seeds. Analysis of gene expression showed that the transcription levels of OsMIR168a's target genes such as OsAGO1a, OsAGO1b and OsAGO1d were elevated significantly in the OsMIR168a mutants. Intriguingly, OsAGO18, a member of a new AGO clade that is conserved in monocots, was confirmed to be a target of OsMIR168a not only by informatic prediction but also by expression analysis and a cell-based cleavage assay in the OsMIR168a mutants. Many protein-coding genes and miRNAs showed differential expression in the OsMIR168a mutants, suggesting OsMIR168a exerts a major transcriptional regulatory role, likely through its potential target genes such as OsAGO1s and OsAGO18. KEGG enrichment analysis of these differentially expressed genes pointed to OsMIR168a's involvement in important processes such as plant hormone signalling transduction and plant-pathogen interaction. These data collectively support that the complex regulation module of OsMIR168a-OsAGO1/OsAGO18-miRNAs-target genes contributes to agronomically important traits, which sheds light on miRNA-mediated crop breeding.
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