Evidence map›Paper›PMID 34555082›Full record

ArticlePloS one2021

Guiding cell adhesion and motility by modulating cross-linking and topographic properties of microgel arrays.

Janine Riegert, Alexander Töpel, Jana Schieren, Renee Coryn, Stella Dibenedetto, Dominik Braunmiller, Kamil Zajt, Carmen Schalla, Stephan Rütten, Martin Zenke and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Janine RiegertDept. of Cell Biology, Institute of Biomedical Engineering, RWTH Aachen University, Aachen, Germany.
Alexander TöpelFunctional and Interactive Polymers, Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, Aachen, Germany.ORCID 0000-0002-6801-2222
Jana SchierenDept. of Cell Biology, Institute of Biomedical Engineering, RWTH Aachen University, Aachen, Germany.
Renee CorynDept. of Cell Biology, Institute of Biomedical Engineering, RWTH Aachen University, Aachen, Germany.
Stella DibenedettoDept. of Cell Biology, Institute of Biomedical Engineering, RWTH Aachen University, Aachen, Germany.
Dominik BraunmillerFunctional and Interactive Polymers, Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, Aachen, Germany.
Kamil ZajtDept. of Cell Biology, Institute of Biomedical Engineering, RWTH Aachen University, Aachen, Germany.ORCID 0000-0001-6280-3464
Carmen SchallaDept. of Cell Biology, Institute of Biomedical Engineering, RWTH Aachen University, Aachen, Germany.
Stephan RüttenElectron Microscopy Facility, Institute of Pathology, RWTH Aachen University, Aachen, Germany.
Martin ZenkeDept. of Cell Biology, Institute of Biomedical Engineering, RWTH Aachen University, Aachen, Germany.ORCID 0000-0002-1107-3251
Andrij PichFunctional and Interactive Polymers, Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, Aachen, Germany.
Antonio SechiDept. of Cell Biology, Institute of Biomedical Engineering, RWTH Aachen University, Aachen, Germany.ORCID 0000-0002-1374-1794
RWTH Aachen University · DEDWI – Leibniz Institute for Interactive Materials · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Biomaterial-driven modulation of cell adhesion and migration is a challenging aspect of tissue engineering. Here, we investigated the impact of surface-bound microgel arrays with variable geometry and adjustable cross-linking properties on cell adhesion and migration. We show that cell migration is inversely correlated with microgel array spacing, whereas directionality increases as array spacing increases. Focal adhesion dynamics is also modulated by microgel topography resulting in less dynamic focal adhesions on surface-bound microgels. Microgels also modulate the motility and adhesion of Sertoli cells used as a model for cell migration and adhesion. Both focal adhesion dynamics and speed are reduced on microgels. Interestingly, Gas2L1, a component of the cytoskeleton that mediates the interaction between microtubules and microfilaments, is dispensable for the regulation of cell adhesion and migration on microgels. Finally, increasing microgel cross-linking causes a clear reduction of focal adhesion turnover in Sertoli cells. These findings not only show that spacing and rigidity of surface-grafted microgels arrays can be effectively used to modulate cell adhesion and motility of diverse cellular systems, but they also form the basis for future developments in the fields of medicine and tissue engineering.

Indexed as

Cell AdhesionCell MovementFocal AdhesionsSertoli CellsAnimalsCross-Linking ReagentsMaleMicrogelsRatsSurface PropertiesTissue EngineeringCross-Linking ReagentsMicrogels

Identifiers

PMID34555082
PMCPMC8460069
OpenAlexW3201177433

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.