Evidence map›Paper›PMID 34554377›Full record

ArticleIn vitro cellular & developmental biology. Animal2021

Integrated analysis of mRNA and long non-coding RNA expression profiles reveals the potential roles of lncRNA-mRNA network in carp macrophage immune regulation.

Shuai Liu, Ting Yu, Yuanyuan Zhang, Chenyuan Pan, Ling Cai, Ming Yang

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In one paragraph

Article in In vitro cellular & developmental biology. Animal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.6field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Shuai Liu *School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, China.
Ting Yu *School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, China.
Yuanyuan ZhangSchool of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, China.
Chenyuan PanSchool of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, China.
Ling CaiThird Institute of Oceanography, Ministry of Natural Resources, Xiamen, 361005, China. cailing@tio.org.cn.
Ming YangSchool of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, China. mingyang@shu.edu.cn.ORCID http://orcid.org/0000-0001-6417-4235
Shanghai University · CNMinistry of Natural Resources · CN

Funding

National Natural Science Foundation of China No.31971523Natural Science Foundation of Fujian Province No.2018J05066
6 · The paper itself

Abstract

Long non-coding RNAs (lncRNAs) have emerged as a hot topic in research as mounting evidence has indicated their transcriptional or post-transcriptional regulatory potential in multiple biological processes. Previous studies have revealed the involvement of lncRNAs in the immunoregulation of mammalian macrophages by changing mRNA expression; however, studies on the lncRNAs in fish macrophages and their potential roles in the immune system remain unknown. Primary macrophages were isolated from the head kidney (HK) of red common carp (Cyprinus carpio) and high-throughput lncRNA-mRNA sequencing was performed using the Illumina HiSeq platform. The results revealed that the most highly expressed mRNAs in primary HK macrophages were mainly involved in immune-related signal pathways. Furthermore, the most enriched immune-related GO term and KEGG pathway of the mRNAs were "immune system development" and "chemokine signaling pathway," respectively. A total of 20,333 lncRNAs, composed of 10,512 known and 9821 novel lncRNAs, were identified, and functional enrichment analysis of the lncRNA-mRNA network indicated that the expressed lncRNAs in primary HK macrophages could be associated with the regulation of multiple immune-related signaling pathways. In addition, the expressions of several selected lncRNAs and their related mRNAs were determined in carp macrophages following a 6-h exposure to lipopolysaccharide (LPS) and Poly(I: C), the results of which confirmed the co-expression regulation of lncRNAs and target mRNAs in the immune response of carp macrophages. These results suggest the correlative of the lncRNA-mRNA network in fish macrophage immune response, which may further affect the cross-talk of various signaling pathways by interaction with other network genes. Here, we provided fundamental data about the transcriptome profiles of primary HK macrophages from red common carp by analysis of the lncRNA-mRNA network, and ultimately suggest the potential roles of lncRNA-mRNA networks in immune regulation in teleost fish.

Indexed as

ImmunityAnimalsCarpsGene Expression RegulationHigh-Throughput Nucleotide SequencingMacrophagesReal-Time Polymerase Chain ReactionRNA, Long NoncodingRNA, MessengerRNA, Long NoncodingRNA, MessengerCommon carpImmune regulationLncRNALncRNA-mRNA networkPrimary macrophages

Identifiers

PMID34554377
OpenAlexW3200177157

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.