Evidence map›Paper›PMID 34554241›Full record

ArticleJournal of molecular cell biology2022

SKAP interacts with Aurora B to guide end-on capture of spindle microtubules via phase separation.

Manjuan Zhang, Fengrui Yang, Wenwen Wang, Najdat Zohbi, Xiwei Wang, Dongmei Wang, Xiaoxuan Zhuang, Zhen Dou, Dan Liu, Xiaoyu Song and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of molecular cell biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Manjuan ZhangMOE Key Laboratory for Cellular Dynamics and Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230027, China.
Fengrui YangMOE Key Laboratory for Cellular Dynamics and Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230027, China.
Wenwen WangMOE Key Laboratory for Cellular Dynamics and Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230027, China.
Najdat ZohbiKeck Center for Organoids Plasticity Control, Morehouse School of Medicine, Atlanta, GA 30310, USA.
Xiwei WangMOE Key Laboratory for Cellular Dynamics and Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230027, China.
Dongmei WangMOE Key Laboratory for Cellular Dynamics and Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230027, China.
Xiaoxuan ZhuangMOE Key Laboratory for Cellular Dynamics and Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230027, China.
Zhen DouMOE Key Laboratory for Cellular Dynamics and Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230027, China.ORCID 0000-0003-3303-9393
Dan LiuMOE Key Laboratory for Cellular Dynamics and Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230027, China.
Xiaoyu SongMOE Key Laboratory for Cellular Dynamics and Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230027, China.
Hadiyah-Nicole GreenKeck Center for Organoids Plasticity Control, Morehouse School of Medicine, Atlanta, GA 30310, USA.
Xing LiuMOE Key Laboratory for Cellular Dynamics and Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230027, China.ORCID 0000-0002-7480-5085
Xuebiao YaoMOE Key Laboratory for Cellular Dynamics and Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230027, China.

Funding

The function of ezrin in stimulus-coupled acid secretionR01DK056292 · NIDDK · UNIVERSITY OF WISCONSIN MADISON · PI YAO, XUEBIAO · 1999 to 2012
$2.5M
Function of ACAP4 in CCL18-stimulated breast cancer metastasisR01CA164133 · NCI · MOREHOUSE SCHOOL OF MEDICINE · PI YAO, XUEBIAO · 2012 to 2016
$1.4M
FUNCTION OF MST4-EZRIN-ACAP4 SIGNALING IN GASTRIC PARIETAL CELL SECRETION AND HOMEOSTASISR01DK115812 · NIDDK · MOREHOUSE SCHOOL OF MEDICINE · PI YAO, XUEBIAO · 2017 to 2019
$967k
NCI NIH HHS R01 CA164133NIDDK NIH HHS R01 DK056292NIDDK NIH HHS R01 DK115812NIH HHS CA164133
6 · The paper itself

Abstract

Chromosome segregation in mitosis is orchestrated by the dynamic interactions between the kinetochore and spindle microtubules. Our recent studies show that mitotic motor CENP-E cooperates with SKAP and forms a link between kinetochore core MIS13 complex and spindle microtubule plus-ends to achieve accurate chromosome alignment in mitosis. However, it remains elusive how SKAP regulates kinetochore attachment from lateral association to end-on attachment during metaphase alignment. Here, we identify a novel interaction between Aurora B and SKAP that orchestrates accurate interaction between the kinetochore and dynamic spindle microtubules. Interestingly, SKAP spontaneously phase-separates in vitro via weak, multivalent interactions into droplets with fast internal dynamics. SKAP and Aurora B form heterogeneous coacervates in vitro, which recapitulate the dynamics and behavior of SKAP comets in vivo. Importantly, SKAP interaction with Aurora B via phase separation is essential for accurate chromosome segregation and alignment. Based on those findings, we reason that SKAP-Aurora B interaction via phase separation constitutes a dynamic pool of Aurora B activity during the lateral to end-on conversion of kinetochore-microtubule attachments to achieve faithful cell division.

Indexed as

Cell Cycle ProteinsMicrotubule-Associated ProteinsAurora Kinase BChromosome SegregationHeLa CellsHumansKinetochoresMicrotubulesMitosisAURKB protein, humanAurora Kinase BCell Cycle ProteinsKNSTRN protein, humanMicrotubule-Associated ProteinsAurora Bkinase activitymitosisphase separationSKAP

Identifiers

PMID34554241
PMCPMC8800532

What OpenQuestion holds

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LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.