Evidence map›Paper›PMID 34553319›Full record

ArticleCell stress & chaperones2021

Temperature response of enriched pre-pubertal caprine male germline stem cells in vitro.

Shiva P Singh, Suresh D Kharche, Manisha Pathak, Yogesh K Soni, Kumaresan Gururaj, Atul K Sharma, Manoj K Singh, Manmohan S Chauhan

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell stress & chaperones, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Shiva P SinghAnimal Physiology and Reproduction Division, ICAR-Central Institute for Research On Goats, Makhdoom, Farah, 281 122, Mathura, Uttar Pradesh, India. shiva.singh@icar.gov.in.ORCID https://orcid.org/0000-0002-3554-8821
Suresh D KharcheAnimal Physiology and Reproduction Division, ICAR-Central Institute for Research On Goats, Makhdoom, Farah, 281 122, Mathura, Uttar Pradesh, India.ORCID https://orcid.org/0000-0003-4392-2569
Manisha PathakAnimal Physiology and Reproduction Division, ICAR-Central Institute for Research On Goats, Makhdoom, Farah, 281 122, Mathura, Uttar Pradesh, India.ORCID https://orcid.org/0000-0002-5534-237X
Yogesh K SoniAnimal Physiology and Reproduction Division, ICAR-Central Institute for Research On Goats, Makhdoom, Farah, 281 122, Mathura, Uttar Pradesh, India.ORCID https://orcid.org/0000-0001-5303-1829
Kumaresan GururajAnimal Health Division, ICAR-Central Institute for Research On Goats, Makhdoom, Farah, 281 122, Mathura, Uttar Pradesh, India.ORCID https://orcid.org/0000-0002-1461-1692
Atul K SharmaAnimal Physiology and Reproduction Division, ICAR-Central Institute for Research On Goats, Makhdoom, Farah, 281 122, Mathura, Uttar Pradesh, India.ORCID https://orcid.org/0000-0002-1808-3997
Manoj K SinghAnimal Genetics and Breeding Division, ICAR-Central Institute for Research On Goats, Makhdoom, Farah, 281 122, Mathura, Uttar Pradesh, India.ORCID https://orcid.org/0000-0003-0118-3614
Manmohan S ChauhanICAR-National Dairy Research Institute, Karnal, 132001, Haryana, India.
Central Institute for Research on Goats · INNational Dairy Research Institute · IN

Funding

department of biotechnology , ministry of science and technology BT/PR27544/AAQ/1/715/2018
6 · The paper itself

Abstract

The present study aims to evaluate culture temperature-dependent variation in survival, growth characteristics and expression of stress, pluripotency, apoptosis, and adhesion markers in enriched caprine male germline stem cells (cmGSCs). For this, testes from pre-pubertal bucks (4-5 months; n = 4) were used to isolated cells by a two-step enzymatic digestion method. After enrichment of cmGSCs by multiple methods (differential platting, Percoll density gradient centrifugation, and MACS), viability of CD90

Indexed as

AnimalsCell DifferentiationCell ProliferationCell SurvivalGene Expression Regulation, DevelopmentalGerm CellsGoatsHSP72 Heat-Shock ProteinsIntegrin beta ChainsMalePluripotent Stem CellsSertoli CellsSuperoxide Dismutase-1TemperatureTestisHSP72 Heat-Shock ProteinsIntegrin beta ChainsSuperoxide Dismutase-1Culture characteristicsEnrichmentMale germline stem cellsPre-pubertal goatsTemperature response

Identifiers

PMID34553319
PMCPMC8578525
OpenAlexW3200277748

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.