ArticleACS photonics2021
Tunable Wide-Field Illumination and Single-Molecule Photoswitching with a Single MEMS Mirror.
Article in ACS photonics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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Who cites it
3 citing papers in PubMed.
- Benchmarking Thiolate-Driven Photoswitching of Cyanine Dyes.The journal of physical chemistry. B · 2023Article
- Miniaturized structured illumination microscopy using two 3-axis MEMS micromirrors.Biomedical optics express · 2022Article
- Single-molecule counting applied to the study of GPCR oligomerization.Biophysical journal · 2022Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Homogeneous illumination in single-molecule localization microscopy (SMLM) is key for the quantitative analysis of super-resolution images. Therefore, different approaches for flat-field illumination have been introduced as alternative to the conventional Gaussian illumination. Here, we introduce a single microelectromechanical systems (MEMS) mirror as a tunable and cost-effective device for adapting wide-field illumination in SMLM. In flat-field mode the MEMS allowed for consistent SMLM metrics across the entire field of view. Employing single-molecule photoswitching, we developed a simple yet powerful routine to benchmark different illumination schemes on the basis of local emitter brightness and ON-state lifetime. Moreover, we propose that tuning the MEMS beyond optimal flat-field conditions enables to study the kinetics of photoswitchable fluorophores within a single acquisition.
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Registered trials
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