Evidence map›Paper›PMID 34552632›Full record

ArticleStem cells international2021

Changes in Methylation Patterns of Tumor Suppressor Genes during Extended Human Embryonic Stem Cell Cultures.

Kyung Min Kang, Jeoung Eun Lee, Ji Eun Park, Hyunjin Kim, Hee Yeon Jang, Minyeon Go, Dong Ryul Lee, Sung Han Shim

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells international, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 92% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Kyung Min KangCenter for Genome Diagnostics, CHA Biotech Inc., Seoul 06135, Republic of Korea.ORCID https://orcid.org/0000-0001-5607-5560
Jeoung Eun LeeCHA Advanced Research Institute, CHA University, Seongnam, Gyunggi-do 13488, Republic of Korea.
Ji Eun ParkCenter for Genome Diagnostics, CHA Biotech Inc., Seoul 06135, Republic of Korea.
Hyunjin KimCenter for Genome Diagnostics, CHA Biotech Inc., Seoul 06135, Republic of Korea.
Hee Yeon JangCenter for Genome Diagnostics, CHA Biotech Inc., Seoul 06135, Republic of Korea.
Minyeon GoCenter for Genome Diagnostics, CHA Biotech Inc., Seoul 06135, Republic of Korea.
Dong Ryul LeeDepartment of Biomedical Science, College of Life Science, CHA University, Seongnam 13488, Republic of Korea.
Sung Han ShimDepartment of Biomedical Science, College of Life Science, CHA University, Seongnam 13488, Republic of Korea.ORCID https://orcid.org/0000-0002-9814-5448
CHA University · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

While studies on embryonic stem cells have been actively conducted, little is known about the epigenetic mechanisms in human embryonic stem cells (hESCs) in extended culture systems. Here, we investigated whether CpG island (CGI) methylation patterns of 24 tumor suppressor genes could be maintained during extended hESC cultures. In total, 10 hESC lines were analyzed. For each cell line, genomic DNA was extracted from early and late passages of cell cultures. CGI methylation levels of 24 tumor suppressor genes were analyzed using methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA), pyrosequencing, and real-time polymerase chain reaction (PCR). Different CGI methylation patterns of

Identifiers

PMID34552632
PMCPMC8452414
OpenAlexW3197806915

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.