ArticleFrontiers in cellular neuroscience2021
Codon Optimization of Insect Odorant Receptor Genes May Increase Their Stable Expression for Functional Characterization in HEK293 Cells.
Article in Frontiers in cellular neuroscience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed, 22 citations in OpenAlex.
- Distribution and efficacy of ingested dsRNA targeting tissue-specific genes in the Argentine ant, Linepithema humile.PloS one · 2026Article
- Highly Expressed Odorant Receptor Orthologs Detect the Aggregation Pheromone Lineatin in Trypodendron Ambrosia Beetles.Molecular ecology · 2025Article
- Alternative strategies based on transgenic Drosophila melanogaster for the functional characterization of insect Ionotropic Receptors.Biological research · 2025Article
- Roles of sensory receptors in non-sensory organs: the kidney and beyond.Nature reviews. Nephrology · 2025Review
- Eurasian spruce bark beetle detects lanierone using a highly expressed specialist odorant receptor, present in several functional sensillum types.BMC biology · 2024Article
- Functional Characterization Supports Multiple Evolutionary Origins of Pheromone Receptors in Bark Beetles.Molecular biology and evolution · 2024Article
- High-throughput ligand profile characterization in novel cell lines expressing seven heterologous insect olfactory receptors for the detection of volatile plant biomarkers.Scientific reports · 2023Article
- Rational engineering approaches for establishing insect olfaction reporters in yeast.Biotechnology notes (Amsterdam, Netherlands) · 2023Article
- Odorant receptor orthologues in conifer-feeding beetles display conserved responses to ecologically relevant odours.Molecular ecology · 2022Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
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Abstract
Insect odorant receptor (OR) genes are routinely expressed in Human Embryonic Kidney (HEK) 293 cells for functional characterization ("de-orphanization") using transient or stable expression. However, progress in this research field has been hampered because some insect ORs are not functional in this system, which may be due to insufficient protein levels. We investigated whether codon optimization of insect OR sequences for expression in human cells could facilitate their functional characterization in HEK293 cells with stable and inducible expression. We tested the olfactory receptor co-receptor (Orco) proteins from the bark beetles
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