ArticleCommunications biology2021
Isochoric supercooled preservation and revival of human cardiac microtissues.
Article in Communications biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- Solid Lipid Nanoparticles in Food Applications: Challenges, Optimization Strategies, and Emerging Supercooling Technologies.Comprehensive reviews in food science and food safety · 2026Review
- A High-Throughput Platform for Measuring and Predicting Vitrification Behavior in Multicomponent Aqueous Solutions.ACS applied materials & interfaces · 2026Article
- A high throughput platform for measuring and predicting vitrification behavior in multicomponent aqueous solutions.bioRxiv : the preprint server for biology · 2026Article
- Article
- Evaluating Isochoric Freezing as a Strategy for Storage of Red Blood Cells.Biotechnology and bioengineering · 2026Article
- Thermodynamic Principles of Emerging Cryopreservation Technologies.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Cryopreservation Strategies to Improve Access to Organ Transplantation.Transplantation · 2026Review
- Anti-Icing Organogel Enables Quasi-Homogeneous Supercooling Preservation of Mouse Hearts.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Combined isochoric processes of freezing and supercooling.NPJ science of food · 2025Article
- Overcoming ice: cutting-edge materials and advanced strategies for effective cryopreservation of biosample.Journal of nanobiotechnology · 2025Review
- Vascular Microphysiological System for Investigating Endothelial Barrier Function During Organ Preservation and Reperfusion.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Predictive Thermodynamics for Isochoric (Constant-Volume) Cryopreservation Systems.The journal of physical chemistry. B · 2025Article
- Beyond the icebox: modern strategies in organ preservation for transplantation.Clinical transplantation and research · 2024Review
- Revealing isochoric water nucleation: a visual study.Scientific reports · 2024Article
- Advances in cardiac tissue engineering and heart-on-a-chip.Journal of biomedical materials research. Part A · 2024Review
- Prototype isochoric preservation device for large organs.Frontiers in bioengineering and biotechnology · 2024Article
- Zebrafish as a high throughput model for organ preservation and transplantation research.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2023Article
- Isochoric Supercooling Organ Preservation System.Bioengineering (Basel, Switzerland) · 2023Article
- An exploratory study on isochoric supercooling preservation of the pig liver.Biochemistry and biophysics reports · 2023Article
- Freezing Biological Time: A Modern Perspective on Organ Preservation.Stem cells translational medicine · 2023Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Low-temperature biopreservation and 3D tissue engineering present two differing routes towards eventual on-demand access to transplantable biologics, but recent advances in both fields present critical new opportunities for crossover between them. In this work, we demonstrate sub-zero centigrade preservation and revival of autonomously beating three-dimensional human induced pluripotent stem cell (hiPSC)-derived cardiac microtissues via isochoric supercooling, without the use of chemical cryoprotectants. We show that these tissues can cease autonomous beating during preservation and resume it after warming, that the supercooling process does not affect sarcomere structural integrity, and that the tissues maintain responsiveness to drug exposure following revival. Our work suggests both that functional three dimensional (3D) engineered tissues may provide an excellent high-content, low-risk testbed to study complex tissue biopreservation in a genetically human context, and that isochoric supercooling may provide a robust method for preserving and reviving engineered tissues themselves.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.