ArticleScientific reports2021
Comparison of DNA and RNA sequencing of total nucleic acids from human cervix for metagenomics.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 21 citations in OpenAlex.
- Viruses in the female lower reproductive tract: a systematic descriptive review of metagenomic investigations.NPJ biofilms and microbiomes · 2024Pooled it
- Microbiome research in liver transplantation.Hepatobiliary surgery and nutrition · 2026Article
- Benchmarking methods for extracting microbial signal from host-dominated metatranscriptomes.Briefings in bioinformatics · 2026Article
- Unveiling the Viral Landscape in Gastric Cancer.Current microbiology · 2026Article
- Clinical metagenomics for diagnosis and surveillance of viral pathogens.Nature reviews. Microbiology · 2026Review
- Limitations and optimizations of cellular lineages tracking.PLoS computational biology · 2025Article
- Development and performance evaluation of a clinical metagenomics approach for identifying pathogens in the whole blood from patients with undifferentiated fever.Frontiers in cellular and infection microbiology · 2025Article
- Exploring vaginal microbiome: from traditional methods to metagenomic next-generation sequencing-a systematic review.Frontiers in microbiology · 2025Review
- Non-Targeted RNA Sequencing: Towards the Development of Universal Clinical Diagnosis Methods for Human and Veterinary Infectious Diseases.Veterinary sciences · 2024Review
- Microbiota changes: the unseen players in cervical cancer progression.Frontiers in microbiology · 2024Review
- Molecular diagnostic approaches for SARS-CoV-2 detection and pathophysiological consequences.Molecular biology reports · 2023Review
- Microbiomes, Their Function, and Cancer: How Metatranscriptomics Can Close the Knowledge Gap.International journal of molecular sciences · 2023Review
- Analysis of the cervical microbiome in women from the German national cervical cancer screening program.Journal of cancer research and clinical oncology · 2023Article
- Metagenomic Sequencing in the ICU for Precision Diagnosis of Critical Infectious Illnesses.Critical care (London, England) · 2023Review
- Effect of Antibiotic Eye Drops on the Nasal Microbiome in Healthy Subjects-A Pilot Study.Antibiotics (Basel, Switzerland) · 2023Article
- Clinical metagenomics-challenges and future prospects.Frontiers in microbiology · 2023Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although metagenomics and metatranscriptomics are commonly used to identify bacteria and viruses in human samples, few studies directly compare these strategies. We wished to compare DNA and RNA sequencing of bacterial and viral metagenomes and metatranscriptomes in the human cervix. Total nucleic acids from six human cervical samples were subjected to DNA and RNA sequencing. The effect of DNase-treatment before reverse transcription to cDNA were also analyzed. Similarities and differences in the metagenomic findings with the three different sequencing approaches were evaluated. A higher proportion of human sequences were detected by DNA sequencing (93%) compared to RNA sequencing without (76%) and with prior DNase-treatment (11%). On the contrary, bacterial sequences increased 17 and 91 times. However, the number of detected bacterial genera were less by RNA sequencing, suggesting that only a few contribute to most of the bacterial transcripts. The viral sequences were less by RNA sequencing, still twice as many virus genera were detected, including some RNA viruses that were missed by DNA sequencing. Metatranscriptomics of total cDNA provided improved detection of mainly transcribed bacteria and viruses in cervical swabs as well as detection of RNA viruses, compared to metagenomics.
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