ArticleJournal of clinical microbiology2021
Evaluation of Nanopore Sequencing as a Diagnostic Tool for the Rapid Identification of Mycoplasma bovis from Individual and Pooled Respiratory Tract Samples.
Article in Journal of clinical microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 47 citations in OpenAlex.
- Integrated meta-analysis and sentinel surveillance: Global prevalence and risk factors forOne health (Amsterdam, Netherlands) · 2026Article
- Bovine Respiratory Mycoplasmas and the Commensal-Pathogen Continuum: A Systematic Review of Vaccines and Diagnostic Approaches.Animals : an open access journal from MDPI · 2026Review
- Exploring the functional microbiome of pigs within the porcine respiratory disease complex: viral-bacterial co-infections and virulence factor profiling.Microbiology spectrum · 2026Article
- Nanopore Sequencing in Veterinary Pathogen Detection: A Review of Technologies and Applications.Veterinary sciences · 2026Review
- Whole genome sequencing in animal health: applications, challenges, and future directions.BMC veterinary research · 2026Review
- Investigating bronchoalveolar lavage fluid cytology in relation to pathogen identification and consolidation depth in calves.Journal of veterinary internal medicine · 2026Article
- Bulk tank milk ELISA as screening test for Mycoplasma bovis: herd classification based on serology and PCR testing of different age groups.Irish veterinary journal · 2026Article
- Diagnostic sensitivity and specificity of metagenomic sequencing and qPCR for detection of viruses associated with bovine respiratory disease estimated using Bayesian latent class models.Frontiers in veterinary science · 2026Article
- Accuracy of two Sepsityper MALDI-TOF MS methods for bacterial identification in bloodstream infections in dogs, foals, and calves using Bayesian latent class model.The veterinary quarterly · 2025Article
- Evaluating the Diagnostic Performance of Long-Read Metagenomic Sequencing Compared to Culture and Antimicrobial Susceptibility Testing for Detection of Bovine Respiratory Bacteria and Indicators of Antimicrobial Resistance.Antibiotics (Basel, Switzerland) · 2025Article
- Identifying Infectious Agents in Snakes (Boidae and Pythonidae) with and Without Respiratory Disease.Animals : an open access journal from MDPI · 2025Article
- Characterization of the enteric virome of clinically healthy pigs around weaning on commercial farms in the Netherlands using next generation sequencing and qPCR.Porcine health management · 2025Article
- Coronaviruses in diarrheic pigeons and the first determination of the complete genome of a new pigeon gammacoronavirus.Scientific reports · 2025Article
- Pathogen and Hemocyte Dynamics in Three Apiaries Across a Bee Season.Transboundary and emerging diseases · 2025Article
- A Spanish Porcine Reproductive and Respiratory Syndrome Virus 1 Strain Is Highly Virulent in Pigs Under Experimental Conditions.Transboundary and emerging diseases · 2025Article
- Porcine ear necrosis in nursery piglets is preceded by oral manipulations of the ear.Porcine health management · 2024Article
- New insights into honey bee viral and bacterial seasonal infection patterns using third-generation nanopore sequencing on honey bee haemolymph.Veterinary research · 2024Article
- Recombinant Viruses from theViruses · 2024Article
- Laboratory tools for the direct detection of bacterial respiratory infections and antimicrobial resistance: a scoping review.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2024Article
- Diagnostic performance of Mycoplasmopsis bovis antibody ELISA tests on bulk tank milk from dairy herds.BMC veterinary research · 2024Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapid identification of Mycoplasma bovis infections in cattle is a key factor to guide antimicrobial therapy and biosecurity measures. Recently, Nanopore sequencing became an affordable diagnostic tool for both clinically relevant viruses and bacteria, but the diagnostic accuracy for M. bovis identification is undocumented. Therefore, in this study Nanopore sequencing was compared to rapid identification of M. bovis with matrix-assisted laser desorption ionization-time of flight mass spectrometry (RIMM) and a triplex real-time PCR assay in a Bayesian latent class model (BLCM) for M. bovis in bronchoalveolar lavage fluid (BALf) samples obtained from calves. In practice, pooling of samples is often used to save money, but the influence on diagnostic accuracy has not been described for M. bovis. Therefore, a convenience sample of 17 pooled samples containing 5 individual BALf samples per farm was analyzed as well. The results for the pooled samples were compared with those for the individual samples to determine sensitivity and specificity. The BLCM showed good sensitivity (77.3% [95% credible interval, 57.8 to 92.8%]) and high specificity (97.4% [91.5 to 99.7%]) for Nanopore sequencing, compared to RIMM (sensitivity, 93.0% [76.8 to 99.5%]; specificity, 91.3% [82.5 to 97.0%]) and real-time PCR (sensitivity, 94.6% [89.7 to 97.7%]; specificity, 86.0% [76.1 to 93.6%]). Sensitivity and specificity of pooled analysis for M. bovis were 85.7% (95% confidence interval, 59.8 to 111.6%) and 90.0% (71.4 to 108.6%%), respectively, for Nanopore sequencing and 100% (100% to 100%) and 88.9% (68.4 to 109.4%) for RIMM. In conclusion, Nanopore sequencing is a rapid, reliable tool for the identification of M. bovis. To reduce costs and increase the chance of M. bovis identification, pooling of 5 samples for Nanopore sequencing and RIMM is possible.
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