Evidence map›Paper›PMID 34550443›Full record

ArticleInternational journal of legal medicine2021

Development and validation of novel 8-dye short tandem repeat multiplex system for forensic applications.

Bowei Jiang, Weifeng Qu, Feng Wang, Liming Zhang, Haibo Rong, Jienan Li, Dan Wen, Moutanou Modeste Judes Zeye, Wei He, Chudong Wang and 7 more

Abstract read
PubMed Publisher
In one paragraph

Article in International journal of legal medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Validation of the PowerPlexInternational journal of legal medicine · 2024
    Article
  2. Article
  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Bowei JiangThe First Research Institute of the Ministry of Public Security of P.R.C, Haidian District, No.1. Shouti South Road, Beijing, 100044, China.
Weifeng QuDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, Hunan Province, No. 172. Tongzipo Road, Changsha, 410013, China.
Feng WangThe First Research Institute of the Ministry of Public Security of P.R.C, Haidian District, No.1. Shouti South Road, Beijing, 100044, China.
Liming ZhangThe First Research Institute of the Ministry of Public Security of P.R.C, Haidian District, No.1. Shouti South Road, Beijing, 100044, China.
Haibo RongThe First Research Institute of the Ministry of Public Security of P.R.C, Haidian District, No.1. Shouti South Road, Beijing, 100044, China.
Jienan LiDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, Hunan Province, No. 172. Tongzipo Road, Changsha, 410013, China.
Dan WenDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, Hunan Province, No. 172. Tongzipo Road, Changsha, 410013, China.
Moutanou Modeste Judes ZeyeDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, Hunan Province, No. 172. Tongzipo Road, Changsha, 410013, China.
Wei HeDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, Hunan Province, No. 172. Tongzipo Road, Changsha, 410013, China.
Chudong WangDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, Hunan Province, No. 172. Tongzipo Road, Changsha, 410013, China.
Hao XingDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, Hunan Province, No. 172. Tongzipo Road, Changsha, 410013, China.
Tao ZhangThe First Research Institute of the Ministry of Public Security of P.R.C, Haidian District, No.1. Shouti South Road, Beijing, 100044, China.
Chuan JinThe First Research Institute of the Ministry of Public Security of P.R.C, Haidian District, No.1. Shouti South Road, Beijing, 100044, China.
Li ChenThe First Research Institute of the Ministry of Public Security of P.R.C, Haidian District, No.1. Shouti South Road, Beijing, 100044, China.
Ying LiuDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, Hunan Province, No. 172. Tongzipo Road, Changsha, 410013, China.
Jifeng CaiDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, Hunan Province, No. 172. Tongzipo Road, Changsha, 410013, China.
Lagabaiyila ZhaDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, Hunan Province, No. 172. Tongzipo Road, Changsha, 410013, China. 40409716@qq.com.ORCID http://orcid.org/0000-0002-7900-122X

Funding

national natural science foundation of china 81871533natural science foundation of hunan province 2020JJ4779
6 · The paper itself

Abstract

DNA profiling of short tandem repeats (STRs) is the primary method for genotyping forensic samples. However, degraded DNA and trace samples are still major problems for commercial 5- or 6-dye STR kits. In order to improve the performance of this method, we developed a novel 8-dye STR multiplex system containing 18 autosomal loci (D3S1358, D1S1656, TPOX, D16S539, vWA, D6S1043, D2S1338, CSF1PO, D19S433, D7S820, FGA, D8S1179, D5S818, D13S317, TH01, D21S11, D12S391, and PentaD) and the sex-determining locus Amelogenin, with all fragments smaller than 330 bases. Validation was carried out as recommended by the Scientific Working Group on DNA Analysis Methods. The results showed that complete profiles were obtainable when the input DNA was as low as 0.0625 ng. Full profiles were obtained even in the presence of inhibitors such as humic acid (< 300 ng/μl), hematin (< 100 μM), and indigo (0.01%). The 8-dye STR multiplex system also showed good performance in the detection degraded DNA samples. These results indicate that the 8-dye STR multiplex system is suitable for human DNA genotyping, including for difficult forensic materials.

Indexed as

DNA FingerprintingMicrosatellite RepeatsAmelogeninDNAGene FrequencyGenetics, PopulationHumansAmelogeninDNADevelopmental validationGA118-24B genetic analyzerSTR; 8-dye STR multiplex system

Identifiers

PMID34550443

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.