Evidence map›Paper›PMID 34550010›Full record

ArticleMicrobiology spectrum2021

Characterization of Phosphopantetheinyl Hydrolase from Mycobacterium tuberculosis.

Shilpika Pandey, Amrita Singh, Guangli Yang, Felipe B d'Andrea, Xiuju Jiang, Travis E Hartman, John W Mosior, Ronnie Bourland, Ben Gold, Julia Roberts and 7 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology spectrum, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.1field-weighted citation impact, top 54% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 3 institutions in 1 country.

Shilpika PandeyDepartment of Microbiology and Immunology, Weill Cornell Medicinegrid.471410.7, New York, New York, USA.
Amrita SinghDepartment of Microbiology and Immunology, Weill Cornell Medicinegrid.471410.7, New York, New York, USA.
Guangli YangOrganic Synthesis Core, Memorial Sloan Kettering Cancer Centergrid.51462.34, New York, New York, USA.
Felipe B d'AndreaDepartment of Microbiology and Immunology, Weill Cornell Medicinegrid.471410.7, New York, New York, USA.
Xiuju JiangDepartment of Microbiology and Immunology, Weill Cornell Medicinegrid.471410.7, New York, New York, USA.
Travis E HartmanDepartment of Medicine, Weill Cornell Medicinegrid.471410.7, New York, New York, USA.
John W MosiorDepartment of Biochemistry and Biophysics, Texas A&M University, College Station, Texas, USA.
Ronnie BourlandDepartment of Biochemistry and Biophysics, Texas A&M University, College Station, Texas, USA.
Ben GoldDepartment of Microbiology and Immunology, Weill Cornell Medicinegrid.471410.7, New York, New York, USA.
Julia RobertsDepartment of Microbiology and Immunology, Weill Cornell Medicinegrid.471410.7, New York, New York, USA.
Annie GeigerDepartment of Microbiology and Immunology, Weill Cornell Medicinegrid.471410.7, New York, New York, USA.
Su TangDepartment of Biochemistry and Biophysics, Texas A&M University, College Station, Texas, USA.
Kyu RheeDepartment of Medicine, Weill Cornell Medicinegrid.471410.7, New York, New York, USA.
Ouathek OuerfelliOrganic Synthesis Core, Memorial Sloan Kettering Cancer Centergrid.51462.34, New York, New York, USA.
James C SacchettiniDepartment of Biochemistry and Biophysics, Texas A&M University, College Station, Texas, USA.
Carl F NathanDepartment of Microbiology and Immunology, Weill Cornell Medicinegrid.471410.7, New York, New York, USA.ORCID 0000-0002-7744-8896
Kristin Burns-HuangDepartment of Microbiology and Immunology, Weill Cornell Medicinegrid.471410.7, New York, New York, USA.ORCID 0000-0002-8202-3884
Cornell University · USTexas A&M University · USMemorial Sloan Kettering Cancer Center · US

Funding

X-RAY CRYSTALLOGRAPHYP30CA008748 · NCI · SLOAN-KETTERING INSTITUTE FOR CANCER RES · PI Hongbiao Carl Lekaye · 1985 to 2026
$347.4M
Vulnerabilities in Mycobacterial Cell-Wall BiogenesisP01AI095208 · NIAID · UNIVERSITY OF CALIFORNIA-IRVINE · PI SACCHETTINI, JAMES C · 2012 to 2022
$19.8M
Organic Synthesis at the Service of Biology and MedicineR50CA243895 · NCI · SLOAN-KETTERING INST CAN RESEARCH · PI Ouathek Ouerfelli · 2019 to 2026
$1.9M
Mycobacterial Phosphopantetheine Hydrolase: Potential for Salvage of Coenzyme AR21AI138939 · NIAID · WEILL MEDICAL COLL OF CORNELL UNIV · PI NATHAN, CARL FRANCIS · 2018 to 2019
$466k
NCI NIH HHS P30 CA008748NCI NIH HHS R50 CA243895NIAID NIH HHS P01 AI095208NIAID NIH HHS R21 AI138939
6 · The paper itself

Abstract

Phosphopantetheinyl hydrolase, PptH (Rv2795c), is a recently discovered enzyme from Mycobacterium tuberculosis that removes 4'-phosphopantetheine (Ppt) from holo-carrier proteins (CPs) and thereby opposes the action of phosphopantetheinyl transferases (PPTases). PptH is the first structurally characterized enzyme of the phosphopantetheinyl hydrolase family. However, conditions for optimal activity of PptH have not been defined, and only one substrate has been identified. Here, we provide biochemical characterization of PptH and demonstrate that the enzyme hydrolyzes Ppt

Indexed as

AnimalsBacterial ProteinsCell WallFemaleHumansLipidsMiceMice, Inbred C57BLMycobacterium tuberculosisPantetheinePhosphoric Diester HydrolasesProtein Processing, Post-TranslationalTuberculosisVirulence4'-phosphopantetheineBacterial ProteinsLipidsPantetheinePhosphoric Diester HydrolasespptH protein, Mycobacterium tuberculosiscarrier proteinCoA salvagedephosphopantetheinylationmetallophosphodiesteraseMycobacterium tuberculosisphosphopantetheinyl hydrolase

Identifiers

PMID34550010
PMCPMC8557913
OpenAlexW3199262762

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.