ArticleThe EMBO journal2021
Molecular diversity of diencephalic astrocytes reveals adult astrogenesis regulated by Smad4.
Article in The EMBO journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
- Single Cell-Type Spatial Proteomics Uncovers Regional Heterogeneity of Astrocytes.Journal of proteome research · 2026Article
- Suppression of astrocyte BMP signaling improves molecular signatures and functional deficits in a fragile X syndrome mouse model.Nature communications · 2026Article
- Astrocyte heterogeneity and gliosis in Huntington's disease: Histopathological insights into striatal and white matter pathology.Histology and histopathology · 2026Review
- Astrocyte heterogeneity in the neocortex: developmental origins, molecular diversity, and functional implications.Frontiers in cell and developmental biology · 2026Review
- Navigating Drug Discovery for Myhre Syndrome: The Complexity of a Multisystemic Rare Disease.American journal of medical genetics. Part C, Seminars in medical genetics · 2025Review
- Astrocyte diversity and subtypes: aligning transcriptomics with multimodal perspectives.EMBO reports · 2025Review
- Article
- Astrocyte heterogeneity reveals region-specific astrogenesis in the white matter.Nature neuroscience · 2025Article
- Dissecting the spatiotemporal diversity of adult neural stem cells.Molecular systems biology · 2024Article
- Astrocytes phenomics as new druggable targets in healthy aging and Alzheimer's disease progression.Frontiers in cellular neuroscience · 2024Review
- Article
- Longitudinal scRNA-seq analysis in mouse and human informs optimization of rapid mouse astrocyte differentiation protocols.Nature neuroscience · 2023Article
- The secreted neuronal signal Spock1 promotes blood-brain barrier development.Developmental cell · 2023Article
- Postnatal expression of CD38 in astrocytes regulates synapse formation and adult social memory.The EMBO journal · 2023Article
- Diverse but unique astrocytic phenotypes during embryonic stem cell differentiation, culturing and development.Communications biology · 2023Article
- Mitochondrial dysfunction compromises ciliary homeostasis in astrocytes.The Journal of cell biology · 2023Article
- Striatal spatial heterogeneity, clustering, and white matter association of GFAPFrontiers in cellular neuroscience · 2023Article
- Dentate gyrus astrocytes exhibit layer-specific molecular, morphological and physiological features.Nature neuroscience · 2022Article
- Astrogenesis in the murine dentate gyrus is a life-long and dynamic process.The EMBO journal · 2022Article
- TGF-β as a Key Modulator of Astrocyte Reactivity: Disease Relevance and Therapeutic Implications.Biomedicines · 2022Review
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Astrocytes regulate brain-wide functions and also show region-specific differences, but little is known about how general and region-specific functions are aligned at the single-cell level. To explore this, we isolated adult mouse diencephalic astrocytes by ACSA-2-mediated magnetic-activated cell sorting (MACS). Single-cell RNA-seq revealed 7 gene expression clusters of astrocytes, with 4 forming a supercluster. Within the supercluster, cells differed by gene expression related to ion homeostasis or metabolism, with the former sharing gene expression with other regions and the latter being restricted to specific regions. All clusters showed expression of proliferation-related genes, and proliferation of diencephalic astrocytes was confirmed by immunostaining. Clonal analysis demonstrated low level of astrogenesis in the adult diencephalon, but not in cerebral cortex grey matter. This led to the identification of Smad4 as a key regulator of diencephalic astrocyte in vivo proliferation and in vitro neurosphere formation. Thus, astrocytes show diverse gene expression states related to distinct functions with some subsets being more widespread while others are more regionally restricted. However, all share low-level proliferation revealing the novel concept of adult astrogenesis in the diencephalon.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.