ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2021
Picrosirius Red Staining: Revisiting Its Application to the Qualitative and Quantitative Assessment of Collagen Type I and Type III in Tendon.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
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81 citing papers in PubMed, 109 citations in OpenAlex.
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- Mesenchymal stem cell derived exosomes and injectable platelet-rich fibrin enhance SDFT healing in a donkey tendonitis model.Scientific reports · 2026Article
- Ovarian Fibrosis Through Comparative Analysis of Collagen Architecture.Molecular reproduction and development · 2026Article
- Growth Hormone-Releasing Peptide-6 (GHRP-6) Ameliorates Post-Infarct Ventricular Remodeling and Systolic Dysfunction in a Model of Permanent Coronary Ligation.Pharmaceuticals (Basel, Switzerland) · 2026Article
- Quantification and characterization of collagen in experimentally induced wounds treated with different types of platelet-rich plasma gel.Cell and tissue banking · 2026Article
- Overcoming challenges in cartilage regeneration: The role of chondrogenic inducers.Bioengineering & translational medicine · 2026Review
- Quantitative Histomorphometric Analysis of Collagen Bundles in Masson's Trichrome Stained Rat (Health science reports · 2026Article
- Endometrial Collagen in Woolly Monkey (Lagothrix l. poeppiggi) and the Uakari (Cacajao calvus).Journal of medical primatology · 2026Article
21 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Collagen has a major role in the structural organization of tendons. Picrosirius red (PSR) staining viewed under polarized light microscopy is the standard method to evaluate the organization of collagen fibers in tissues. It is also used to distinguish between type I and type III collagen in tissue sections. However, accurate analysis and interpretation of PSR images are challenging because of technical factors and historical misconceptions. The aim of this study was to clarify whether collagen types I and III can be distinguished by PSR staining in rat Achilles tendons, using double immunohistochemistry as the positive control. Our findings showed that PSR staining viewed with polarized light microscopy was suitable for qualitative and quantitative assessment of total collagen but was not able to distinguish collagen types. We found it critical to use a polarizing microscope equipped with a rotating stage; tendon section orientation at 45° with respect to crossed polarizers was optimal for the qualitative and quantitative assessment of collagen organization. Immunohistochemistry was superior to PSR staining for detection of collagen type III. We also compared formalin and Bouin solution as fixatives. Both produced similar birefringence, but formalin-fixed tendons provided higher quality histological detail with both hematoxylin-eosin and immunostaining.
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