ArticleJournal of assisted reproduction and genetics2021
Thawing day 3 embryos and culturing to day 5 may be a better method for frozen embryo transfer.
Article in Journal of assisted reproduction and genetics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT05858333 (Pregnancy Outcome of Extended Culture of Day 3 to Day 5 Frozen Thawed Day 3 Embryos Versus Day 5 Frozen-Thawed Embryos), which is not on this map. Cited by 6 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Pregnancy Outcome of Extended Culture of Day 3 to Day 5 Frozen Thawed Day 3 Embryos Versus Day 5 Frozen-Thawed Embryos
Who cites it
6 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The impact of post thaw embryo culture (extended culture) on frozen embryo transfer outcomes: a systematic review and meta-analysis.Journal of assisted reproduction and genetics · 2026Pooled it
- Post-thaw embryo culture and the risk of large-for-gestational-age offspring: a retrospective cohort study.Reproductive health · 2026Article
- Pregnancy Outcomes of Frozen-Thawed Blastocysts versus Blastocysts Derived from Frozen-Thawed Cleavage Embryos: A Retrospective Study.International journal of fertility & sterility · 2025Article
- Extending the culture of cleavage-stage embryos to the blastocyst stage after warming increases the chance of live birth: does it have a regenerative effect?Archives of gynecology and obstetrics · 2023Article
- Reproductive Outcomes of Transferring Blastocysts Derived From Frozen-Thawed Cleavage Embryos: A Systematic Review and Meta-Analysis.Reproductive medicine and biologyReview
- Impact of Previous-day versus Same-day Warming of Cleavage-stage Embryos on Reproductive Outcomes: A Retrospective Cohort Study.Journal of human reproductive sciencesArticle
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeDoes thawing cleavage embryos and culturing them for transfer as blastocysts improve pregnancy and perinatal outcomes compared to transferring thawed blastocysts?
methodsRetrospective, observational cohort study performed at two assisted reproductive technology centers, 2014 to 2020. A total of 450 patients with 463 thawed embryo transfer cycles were divided into 2 groups according to the embryonic developmental stage at cryopreservation and transfer: 231 thawed blastocysts (day 5 group) and 232 thawed cleavage embryos that were cultured for 2 days and transferred as blastocysts (day 3-5 group). The two groups were compared for demographics, routine parameters of IVF treatment, pregnancy rates, and perinatal outcomes.
resultsMultivariable logistic regression analysis for ongoing pregnancy and delivery demonstrated that the day 3-5 group had a greater likelihood of achieving ongoing pregnancy and delivery compared to the day 5 group (OR 1.58, 95%CI 1.062-2.361, p = 0.024). Perinatal outcomes were comparable between the three groups.
conclusionOur results support culturing post-thaw cleavage embryos for 2 days and transferring them as blastocysts to increase chances of ongoing pregnancy and delivery.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.