Evidence map›Paper›PMID 34548637›Full record

ArticleThe Journal of antibiotics2022

Heterologous expression of a natural product biosynthetic gene cluster from Cordyceps militaris.

Yang-Le Gao, Cui Yu, Li Li

Abstract read
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In one paragraph

Article in The Journal of antibiotics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.0field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

  1. Innovative application of CRISPR for eliminating Ustiloxin inLebensmittel-Wissenschaft + [i.e. und] Technologie. Food science + technology. Science + technologie alimentaire · 2024
    Article
  2. Biology · 2022
    Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Yang-Le GaoEngineering Research Center of Industrial Microbiology, College of Life Sciences, Fujian Normal University, Fuzhou, China.
Cui YuEngineering Research Center of Industrial Microbiology, College of Life Sciences, Fujian Normal University, Fuzhou, China.
Li LiEngineering Research Center of Industrial Microbiology, College of Life Sciences, Fujian Normal University, Fuzhou, China. lili@fjnu.edu.cn.
Fujian Normal University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cordyceps is a genus of ascomycete fungi widely used in old Chinese medicine, and many investigations have focus on uncovering their biological activities. Until now, only a few compounds have been identified from Cordyceps, mainly due to their poor yield. So as to make full use of Cordyceps, we used the strategy of genome mining and heterologous expression to discover natural products (NPs) from Cordyceps militaris. Analysis of the genome sequence of Cordyceps militaris CM01 showed the presence of a cryptic gene cluster encoding a highly-reducing polyketide synthetase (HR-PKS), enoyl-reductase (ER) and cytochrome P450. Heterologous expression in Aspergillus nidulans enabled the identification of two new polyketides, cordypyrone A and B. Their structures were determined by 1D and 2D NMR techniques. They showed only modest activities against pathogenic bacteria including methicillin-resistant Staphylococcus aureus (MRSA), Mycobacteria tuberculosis and Bacillus cereus.

Indexed as

Biological ProductsAnti-Bacterial AgentsAspergillus nidulansBacillus cereusChromosome MappingCordycepsCytochrome P-450 Enzyme SystemGenes, FungalMagnetic Resonance SpectroscopyMethicillin-Resistant Staphylococcus aureusMicrobial Sensitivity TestsMultigene FamilyMycobacterium tuberculosisPolyketide SynthasesAnti-Bacterial AgentsBiological ProductsCytochrome P-450 Enzyme SystemPolyketide Synthases

Identifiers

PMID34548637
OpenAlexW3200208806

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.