ArticleNature communications2021
Reconstructing aspects of human embryogenesis with pluripotent stem cells.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 115 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
115 citing papers in PubMed, 177 citations in OpenAlex.
- Generation of eight-cell embryo-like cells from human pluripotent stem cells.Nature protocols · 2026Review
- The making of cells and organs in xenogeneic animal bioreactor.Signal transduction and targeted therapy · 2026Review
- Epigenetic blind spots - the role of DNA methylation dynamics in stem cell-based models of embryogenesis.FEBS letters · 2026Article
- Multiscale Modeling of Embryonic Morphogenesis from Fertilization to Organogenesis.NPJ systems biology and applications · 2026Review
- A single small molecule-based human embryo model reveals V-ATPase requirement in mammalian blastocyst cavitation.Cell research · 2026Article
- Decoding the origins of cellular self-organization for engineered biology.Nature biotechnology · 2026Review
- Cyanine-modified ssODNs enhance CRISPR-Cas9 HDR in stem cell embryo models via chromatin and chemical modulation.Nature communications · 2026Article
- Advantages, limitations, and innovative considerations for established and emerging models of human placental syncytiotrophoblast.Human reproduction update · 2026Review
- Progress in stem cell-based embryo models and their applications in developmental biology and biomedicine.Nature reviews. Molecular cell biology · 2026Review
- Amniogenesis in embryos and stem cell models.Nature cell biology · 2026Review
- Tendon healing in the era of regenerative medicine: literature review.Annals of translational medicine · 2026Review
- New models of implantation: towards a whole better than the sum of parts.Human reproduction (Oxford, England) · 2026Review
- Mechanisms of human germ cell development.Nature reviews. Molecular cell biology · 2026Review
- Trophectoderm-like cells from EPS cells enable generating EPS cell-derived post-implantation embryoids that complete gastrulation.Protein & cell · 2026Article
- A human epiblast model reveals dynamic TGFβ-mediated control of epithelial identity during mammalian epiblast development.Nature cell biology · 2026Article
- Application of CRISPR-Based Epigenome Editing Tools for Engineering Programmable Embryo Models.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Engineering Synthetic PEG Hydrogels to Model Peri-implantation Epiblast Morphogenesis with Tunable Biophysical Properties.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Generation of Porcine Blastoids from Embryonic Stem Cells.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Primed human pluripotent stem cell-derived blastocyst-like cell aggregates with partial lineage specification.Regenerative therapy · 2025Article
- deepBlastoid: a deep learning model for automated and efficient evaluation of human blastoids.Life medicine · 2025Article
55 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Understanding human development is of fundamental biological and clinical importance. Despite its significance, mechanisms behind human embryogenesis remain largely unknown. Here, we attempt to model human early embryo development with expanded pluripotent stem cells (EPSCs) in 3-dimensions. We define a protocol that allows us to generate self-organizing cystic structures from human EPSCs that display some hallmarks of human early embryogenesis. These structures mimic polarization and cavitation characteristic of pre-implantation development leading to blastocyst morphology formation and the transition to post-implantation-like organization upon extended culture. Single-cell RNA sequencing of these structures reveals subsets of cells bearing some resemblance to epiblast, hypoblast and trophectoderm lineages. Nevertheless, significant divergences from natural blastocysts persist in some key markers, and signalling pathways point towards ways in which morphology and transcriptional-level cell identities may diverge in stem cell models of the embryo. Thus, this stem cell platform provides insights into the design of stem cell models of embryogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.