Evidence map›Paper›PMID 34546380›Full record

ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2021

A genome-wide association study reveals that the cytochrome b5 involved in seed reserve mobilization during seed germination in rice.

Zhibo Huang, Jifeng Ying, Liling Peng, Shan Sun, Chengwei Huang, Can Li, Zhoufei Wang, Yongqi He

Abstract read
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In one paragraph

Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

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9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

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3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Progress in the study of functional genes related to direct seeding of rice.Molecular breeding : new strategies in plant improvement · 2023
    Review
  7. Article
  8. A new alleleFrontiers in plant science · 2023
    Article
  9. Article
4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Zhibo Huang *The Laboratory of Seed Science and Technology, Guangdong Key Laboratory of Plant Molecular Breeding, Guangdong Laboratory of Lingnan Modern Agriculture, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China.
Jifeng Ying *State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China.
Liling PengThe Laboratory of Seed Science and Technology, Guangdong Key Laboratory of Plant Molecular Breeding, Guangdong Laboratory of Lingnan Modern Agriculture, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China.
Shan SunThe Laboratory of Seed Science and Technology, Guangdong Key Laboratory of Plant Molecular Breeding, Guangdong Laboratory of Lingnan Modern Agriculture, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China.
Chengwei HuangThe Laboratory of Seed Science and Technology, Guangdong Key Laboratory of Plant Molecular Breeding, Guangdong Laboratory of Lingnan Modern Agriculture, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China.
Can LiThe Laboratory of Seed Science and Technology, Guangdong Key Laboratory of Plant Molecular Breeding, Guangdong Laboratory of Lingnan Modern Agriculture, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China.
Zhoufei WangThe Laboratory of Seed Science and Technology, Guangdong Key Laboratory of Plant Molecular Breeding, Guangdong Laboratory of Lingnan Modern Agriculture, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China. wangzf@scau.edu.cn.ORCID http://orcid.org/0000-0002-8565-9175
Yongqi HeThe Laboratory of Seed Science and Technology, Guangdong Key Laboratory of Plant Molecular Breeding, Guangdong Laboratory of Lingnan Modern Agriculture, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China. hyq@scau.edu.cn.

Funding

national natural science foundation of china 31771889national natural science foundation of china 31901601national natural science foundation of china 31971995national natural science foundation of china 32172052
6 · The paper itself

Abstract

key messageA candidate gene cytochrome b5 for the major QTL qSRMP9 for rice seed reserve mobilization was validated during seed germination using a genome-wide association study approach. Seed reserve mobilization plays important roles in the early seedling growth in rice. However, the genetic basis underlying this process is poorly understood. In this study, the genetic architecture of variation in seed reserve mobilization during seed germination was studied using a genome-wide association study approach in rice. Three quantitative trait loci (QTL) including qSRMP6, qSRMP9, and qSRMP12 for seed reserve mobilization percentage were identified. In which, the candidate gene cytochrome b5 (OsCyb5) for the major QTL qSRMP9 was validated. Disruption of this gene in Oscyb5 mutants reduced the seed reserve mobilization and seedling growth compared with wild-type (WT) in rice. There were no significant differences of grain size, starch, protein and total soluble sugar content in the mature grains between Oscyb5 mutants and WT. However, the α-amylase activity in the germinating seeds of Oscyb5 mutants was significantly decreased compared to that of WT, and then, the starch and sugar mobilization and the glucose accumulation during seed germination were significantly decreased in Oscyb5 mutants. Two elite haplotypes of OsCyb5 associated with the higher seed reserve mobilization percentage and its elite single nucleotide polymorphism variations were mainly existed in the INDICA and AUS accessions. The natural variation of OsCyb5 contributing to seed reserve mobilization might be useful for the future rice breeding.

Indexed as

AllelesCytochromes b5Genetic Association StudiesGerminationHaplotypesOryzaQuantitative Trait LociSeedlingsSeedsCytochromes b5

Identifiers

PMID34546380

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