Evidence map›Paper›PMID 34546074›Full record

ArticlemSystems2021

Predicting the Presence and Abundance of Bacterial Taxa in Environmental Communities through Flow Cytometric Fingerprinting.

Jasmine Heyse, Florian Schattenberg, Peter Rubbens, Susann Müller, Willem Waegeman, Nico Boon, Ruben Props

Abstract read
In one paragraph

Article in mSystems, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
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  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Jasmine HeyseCenter for Microbial Ecology and Technology (CMET), Department of Biochemical and Microbial Technology, Ghent University, Ghent, Belgium.ORCID 0000-0002-8858-9803
Florian SchattenbergDepartment of Environmental Microbiology, Helmholtz Centre for Environmental Researchgrid.7492.8-UFZ, Leipzig, Germany.
Peter RubbensFlanders Marine Institutegrid.426539.f (VLIZ), InnovOcean site, Ostend, Belgium.ORCID 0000-0001-5595-4758
Susann MüllerDepartment of Environmental Microbiology, Helmholtz Centre for Environmental Researchgrid.7492.8-UFZ, Leipzig, Germany.
Willem WaegemanKERMIT, Department of Data Analysis and Mathematical Modelling, Ghent University, Ghent, Belgium.
Nico BoonCenter for Microbial Ecology and Technology (CMET), Department of Biochemical and Microbial Technology, Ghent University, Ghent, Belgium.ORCID 0000-0002-7734-3103
Ruben PropsCenter for Microbial Ecology and Technology (CMET), Department of Biochemical and Microbial Technology, Ghent University, Ghent, Belgium.

Funding

Fonds Wetenschappelijk Onderzoek (FWO) 1221020NFonds Wetenschappelijk Onderzoek (FWO) 1S80618NOnderzoeksprogramma Artificiële Intelligentie (AI) VlaanderenUGent | Bijzonder Onderzoeksfonds UGent (BOF) BOF15/GOA/006
6 · The paper itself

Abstract

Microbiome management research and applications rely on temporally resolved measurements of community composition. Current technologies to assess community composition make use of either cultivation or sequencing of genomic material, which can become time-consuming and/or laborious in case high-throughput measurements are required. Here, using data from a shrimp hatchery as an economically relevant case study, we combined 16S rRNA gene amplicon sequencing and flow cytometry data to develop a computational workflow that allows the prediction of taxon abundances based on flow cytometry measurements. The first stage of our pipeline consists of a classifier to predict the presence or absence of the taxon of interest, with yielded an average accuracy of 88.13% ± 4.78% across the top 50 operational taxonomic units (OTUs) of our data set. In the second stage, this classifier was combined with a regression model to predict the relative abundances of the taxon of interest, which yielded an average

Indexed as

16S rRNA gene amplicon sequencingaquaculturecell sortingflow cytometrymachine learningmicrobial community dynamicsmonitoring

Identifiers

PMID34546074
PMCPMC8547484

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.