Evidence map›Paper›PMID 34545497›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Analysis of the Plant Mitochondrial Transcriptome.

Kevin Baudry, Etienne Delannoy, Catherine Colas des Francs-Small

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Kevin BaudryInstitute of Plant Sciences Paris-Saclay (IPS2), Orsay, France.
Etienne DelannoyInstitute of Plant Sciences Paris-Saclay (IPS2), Orsay, France.
Catherine Colas des Francs-SmallARC Centre of Excellence for Plant Energy Biology, The University of Western Australia, WA Crawley, Australia. catherine.colasdesfrancs-small@uwa.edu.au.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Transcriptome analyses are widely performed, but the analysis of organelle genome expression is often overlooked. In this chapter, we describe three methods to analyse the accumulation, splicing, editing and processing of plant mitochondrial transcript expression: a classical RT-qPCR assay, an RNA-Seq approach with its bioinformatical and statistical analysis pipeline, as well as a useful complementary technique, the Northern-blot analysis, using short biotinylated oligonucleotides as probes.

Indexed as

Gene Expression ProfilingTranscriptomePlantsRNA-SeqRNA SplicingSequence Analysis, RNABiotinylated probeMitochondrial transcriptomeNorthern blottingOrganellar RNA-SeqQuantitative RT-PCRRNA editingRNA processingSplicing

Identifiers

PMID34545497

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.