Evidence map›Paper›PMID 34544937›Full record

ArticleThe Journal of veterinary medical science2021

Bovine leukemia virus genotype surveillance in cattle at a slaughterhouse in Aichi Prefecture, Japan, in 2019 using polymerase chain reaction combined with restriction fragment length polymorphism.

Yuma Sahashi, Masaaki Oshima, Junjiro Yamagishi, Chieko Muramatsu, Kaori Shimizu, Yasuo Inoshima

Abstract read
In one paragraph

Article in The Journal of veterinary medical science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Can Oncogenic Animal Viruses Pose a Threat to Humans?Pathogens (Basel, Switzerland) · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Yuma SahashiNagoya City Meat Hygiene Inspection Laboratory, 1-39 Funami, Minato, Nagoya, Aichi 455-0027, Japan.
Masaaki OshimaNagoya City Meat Hygiene Inspection Laboratory, 1-39 Funami, Minato, Nagoya, Aichi 455-0027, Japan.
Junjiro YamagishiNagoya City Meat Hygiene Inspection Laboratory, 1-39 Funami, Minato, Nagoya, Aichi 455-0027, Japan.
Chieko MuramatsuNagoya City Meat Hygiene Inspection Laboratory, 1-39 Funami, Minato, Nagoya, Aichi 455-0027, Japan.
Kaori ShimizuLaboratory of Food and Environmental Hygiene, Cooperative Department of Veterinary Medicine, Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan.
Yasuo InoshimaLaboratory of Food and Environmental Hygiene, Cooperative Department of Veterinary Medicine, Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Polymerase chain reaction (PCR) combined with restriction fragment length polymorphism (RFLP) is commonly used for genotyping bovine leukemia virus (BLV) in slaughterhouses. However, unclassified BLV genotypes have been sporadically reported. To assess the current status of BLV genetic characterization in cattle, PCR-RFLP was performed on blood samples of 170 cattle (84 Japanese Black, 60 Japanese Black x Holstein, and 26 Holstein) from 17 farms (5 prefectures) at a slaughterhouse in Aichi Prefecture in 2019. A total of 65 samples (38.2%) were BLV positive, and genotype 1 was the most predominant (56/65 samples), followed by genotypes 3 (6 samples) and 5 (1 sample), and two unclassified samples. No relationship between the genotypes and breeds was observed. Sequence and phylogenetic analyses demonstrated that unclassified BLV genotypes clustered with genotype 1 sequences were, therefore, not new genotypes.

Indexed as

Cattle DiseasesEnzootic Bovine LeukosisLeukemia Virus, BovineAbattoirsAnimalsCattleGenotypeJapanPhylogenyPolymerase Chain ReactionPolymorphism, Restriction Fragment Lengthbovine leukemia virusepidemiological analysisgenotypepolymerase chain reaction-restriction fragment length polymorphismsequencing

Identifiers

PMID34544937
PMCPMC8636894

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.