ArticleG3 (Bethesda, Md.)2021
Engineering threshold-based selection systems.
Katherine H Pedone, Vanessa González-Pérez, Luciana E Leopold, Neal R Rasmussen, Channing J Der, Adrienne D Cox, Shawn Ahmed, David J Reiner
Open access · goldAbstract read
In one paragraphArticle in G3 (Bethesda, Md.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registryThe trial behind it
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3 · Its place in the literatureWho cites it
0 citing papers in PubMed, 0 citations in OpenAlex.
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4 · The recordCorrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
5 · Who and what moneyAuthors and funding
8 authors at 2 institutions in 1 country.
Katherine H PedoneLineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA.
Vanessa González-PérezCurriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, NC 27599, USA.
Luciana E LeopoldCurriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, NC 27599, USA.
Neal R RasmussenInstitute of Biosciences and Technology, College of Medicine, Texas A&M Health Science Center, Houston, TX 77030, USA.
Channing J DerLineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA.
Adrienne D CoxLineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA.
Shawn AhmedCurriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, NC 27599, USA.ORCID 0000-0001-6365-2194 David J ReinerLineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA.ORCID 0000-0002-0344-7161 University of North Carolina at Chapel Hill · USTexas A&M Health Science Center · US
Funding
Integrated Training in Cancer Model SystemsT32CA009156 · NCI · UNIV OF NORTH CAROLINA CHAPEL HILL · PI DER, CHANNING J. · 1985 to 2020
$19.7MEnhancing and expanding the CGC Strain CollectionP40OD010440 · OD · UNIVERSITY OF MINNESOTA · PI Aric L Daul, Ann E. Rougvie · 2012 to 2026
$7.5MMechanisms of PAK1 activation, signaling and tumor resistanceR01CA175747 · NCI · UNIV OF NORTH CAROLINA CHAPEL HILL · PI DER, CHANNING J., HAHN, KLAUS M. · 2014 to 2018
$1.8MNovel signals regulate cell fate patterning - Equipment SupplementR01GM121625 · NIGMS · TEXAS A&M UNIVERSITY HEALTH SCIENCE CTR · PI REINER, DAVID · 2017 to 2020
$1.4MNCI NIH HHS R01 CA175747NCI NIH HHS T32 CA009156NIGMS NIH HHS R01 GM121625NIH HHS P40 OD010440
6 · The paper itselfAbstract
Using model organisms to identify novel therapeutic targets is frequently constrained by pre-existing genetic toolkits. To expedite positive selection for identification of novel downstream effectors, we engineered conditional expression of activated CED-10/Rac to disrupt Caenorhabditis elegans embryonic morphogenesis, titrated to 100% lethality. The strategy of engineering thresholds for positive selection using experimental animals was validated with pharmacological and genetic suppression and is generalizable to diverse molecular processes and experimental systems.
Indexed as
Caenorhabditis elegans ProteinsAnimalsCaenorhabditis elegansCaenorhabditis elegans Proteins3ʹUTRBH3-onlyEGL-1EHT 1864EHT 8560NMDnonsense-mediated decayPaksmall GTPaseSMG-1
Identifiers
PMID34544135
PMCPMC8496214
OpenAlexW3179439481
What OpenQuestion holds
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