ArticlePLoS pathogens2021
Phase separation of a plant virus movement protein and cellular factors support virus-host interactions.
Article in PLoS pathogens, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Liquid-liquid phase separation at the frontline of plant-pathogen battles.Plant communications · 2026Review
- ricePSP: a database of rice phase separation-associated proteins.Genome biology · 2025Article
- Plant negative-strand RNA virus phosphoprotein condensates exploit host trafficking and lipid synthesis for viral factory assembly.Science advances · 2025Article
- A plant bunyaviral protein disrupts SERRATE phase separation to modulate microRNA biogenesis during viral pathogenesis.Nature communications · 2025Article
- Engineered Biomolecular Condensates Limit Tobacco Mosaic Virus Accumulation and Symptom Development.Molecular plant pathology · 2025Article
- HSP90 interacts with VP37 to facilitate the cell-to-cell movement of broad bean wilt virus 2.mBio · 2025Article
- Viral condensates formed by Pea enation mosaic virus 2 sequester ribosomal components and suppress translation.Virology · 2025Article
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- Subversion of selective autophagy for the biogenesis of tombusvirus replication organelles inhibits autophagy.PLoS pathogens · 2024Article
- Biomolecular condensates in plant RNA silencing: insights into formation, function, and stress responses.The Plant cell · 2024Review
- Cell Fractionation and the Identification of Host Proteins Involved in Plant-Virus Interactions.Pathogens (Basel, Switzerland) · 2024Review
- Unraveling the Mechanisms of Virus-Induced Symptom Development in Plants.Plants (Basel, Switzerland) · 2023Review
- Abnormal phase separation of biomacromolecules in human diseases.Acta biochimica et biophysica Sinica · 2023Review
- Biomolecular phase separation in stress granule assembly and virus infection.Acta biochimica et biophysica Sinica · 2023Article
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- P1 of turnip mosaic virus interacts with NOD19 for vigorous infection.Frontiers in microbiology · 2023Article
- Molecular Variation and Genomic Function of Citrus Vein Enation Virus.International journal of molecular sciences · 2022Article
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Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Both cellular and viral proteins can undergo phase separation and form membraneless compartments that concentrate biomolecules. The p26 movement protein from single-stranded, positive-sense Pea enation mosaic virus 2 (PEMV2) separates into a dense phase in nucleoli where p26 and related orthologues must interact with fibrillarin (Fib2) as a pre-requisite for systemic virus movement. Using in vitro assays, viral ribonucleoprotein complexes containing p26, Fib2, and PEMV2 genomic RNAs formed droplets that may provide the basis for self-assembly in planta. Mutating basic p26 residues (R/K-G) blocked droplet formation and partitioning into Fib2 droplets or the nucleolus and prevented systemic movement of a Tobacco mosaic virus (TMV) vector in Nicotiana benthamiana. Mutating acidic residues (D/E-G) reduced droplet formation in vitro, increased nucleolar retention 6.5-fold, and prevented systemic movement of TMV, thus demonstrating that p26 requires electrostatic interactions for droplet formation and charged residues are critical for nucleolar trafficking and virus movement. p26 readily partitioned into stress granules (SGs), which are membraneless compartments that assemble by clustering of the RNA binding protein G3BP following stress. G3BP is upregulated during PEMV2 infection and over-expression of G3BP restricted PEMV2 RNA accumulation >20-fold. Deletion of the NTF2 domain that is required for G3BP condensation restored PEMV2 RNA accumulation >4-fold, demonstrating that phase separation enhances G3BP antiviral activity. These results indicate that p26 partitions into membraneless compartments with either proviral (Fib2) or antiviral (G3BP) factors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.