Evidence map›Paper›PMID 34542855›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Analysis of Morphogenesis and Flagellar Assembly During Spermatogenesis in Planarian Flatworms.

Labib Rouhana, Tracy Chong, Phillip A Newmark

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.0field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Regulatory Effects of theInternational journal of molecular sciences · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 3 institutions in 1 country.

Labib RouhanaDepartment of Biological Sciences, Wright State University, Dayton, OH, USA. labib.rouhana@wright.edu.
Tracy ChongHoward Hughes Medical Institute, University of Wisconsin-Madison, Madison, WI, USA.
Phillip A NewmarkHoward Hughes Medical Institute, University of Wisconsin-Madison, Madison, WI, USA.
Howard Hughes Medical Institute · USMorgridge Institute for Research · USWright State University · US

Funding

Stem cell proliferation and differentiation in planariaR01HD043403 · NICHD · UNIVERSITY OF ILLINOIS URBANA-CHAMPAIGN · PI Phillip A Newmark · 2003 to 2026
$5.1M
Discovery of germline genes and regulatory networks in planariansR15HD082754 · NICHD · WRIGHT STATE UNIVERSITY · PI ROUHANA, LABIB · 2014 to 2024
$1.3M
Howard Hughes Medical InstituteNICHD NIH HHS R01 HD043403NICHD NIH HHS R15 HD082754
6 · The paper itself

Abstract

Spermatogenesis is one of the most dramatic cellular differentiation events observed in animals. In particular, spermiogenesis (the final stage of spermatogenesis) involves extensive shedding of cytoplasmic organelles, dramatic nuclear rearrangements, and assembly of long flagellar structures. In planarian flatworms, the spherical nucleus present in round spermatids elongates to produce the filamentous nucleus of mature sperm. Newly formed cortical microtubules participate in cytoskeletal rearrangements observed during spermiogenesis and remain present in sperm. In addition, a pair of flagella assemble at one end of each spermatid in a process that likely involves de novo formation of centrioles. This chapter includes a brief introduction to planarian spermatogenesis and current tools for the analysis of molecular players in this process. Step-by-step protocols for isolating and imaging spermatogenic cells are provided with enough detail to be carried out by newcomers to the field who would like to study this unique organism in the laboratory.

Indexed as

PlanariansAnimalsCell NucleusMaleMicrotubulesMorphogenesisSpermatidsSpermatogenesisSpermatozoaFlagellaManchetteMicrotubulesPlanarianPlatyhelminthesSpermatogenesisSpermiogenesis

Identifiers

PMID34542855
OpenAlexW3200546835

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.