Evidence map›Paper›PMID 34542849›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Imaging the Actin Cytoskeleton in Fixed Budding Yeast Cells.

Cierra N Sing, Emily J Yang, Ryo Higuchi-Sanabria, Liza A Pon, Istvan R Boldogh, Theresa C Swayne

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Yeast-mediated display: probingIranian journal of microbiology · 2025
    Article
  3. Article
  4. Elimination of virus-like particles reduces protein aggregation and extends replicative lifespan inProceedings of the National Academy of Sciences of the United States of America · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Cierra N SingDepartment of Pathology and Cell Biology, Columbia University, College of Physicians and Surgeons, New York, NY, USA.
Emily J YangDepartment of Pathology and Cell Biology, Columbia University, College of Physicians and Surgeons, New York, NY, USA.
Ryo Higuchi-SanabriaLeonard Davis School of Gerontology, University of Southern California, Los Angeles, CA, USA.
Liza A PonConfocal and Specialized Microscopy Shared Resource, Herbert Irving Comprehensive Cancer Center, Columbia University, New York, NY, USA. lap5@cumc.columbia.edu.
Istvan R BoldoghDepartment of Pathology and Cell Biology, Columbia University, College of Physicians and Surgeons, New York, NY, USA.
Theresa C SwayneConfocal and Specialized Microscopy Shared Resource, Herbert Irving Comprehensive Cancer Center, Columbia University, New York, NY, USA.

Funding

Tumor Biology and Microenvironment ProgramP30CA013696 · NCI · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI Parisa Tehranifar · 1985 to 2026
$115.3M
NUTRITIONT32DK007647 · NIDDK · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI Anthony W Ferrante, Lori M Zeltser · 1990 to 2026
$7.4M
Mitochondrial inheritance and quality controlR35GM122589 · NIGMS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI Liza A Pon · 2017 to 2026
$5.1M
The Role of Actin in Cellular AgingR33AG051047 · NIA · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI PON, LIZA A · 2018 to 2020
$1.4M
More than just a load control: cytoskeletal form and function during agingR00AG065200 · NIA · UNIVERSITY OF SOUTHERN CALIFORNIA · PI HIGUCHI-SANABRIA, RYO · 2021 to 2023
$747k
The Role of Actin in Cellular AgingR21AG051047 · NIA · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI PON, LIZA A · 2016 to 2017
$427k
More than just a load control: cytoskeletal form and function during agingK99AG065200 · NIA · UNIVERSITY OF CALIFORNIA BERKELEY · PI HIGUCHI-SANABRIA, RYO · 2020 to 2021
$244k
Mitochondria-cytoskeleton communication and function in agingF31AG055326 · NIA · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI SING, CIERRA NICOLE · 2016 to 2020
$179k
NCI NIH HHS P30 CA013696NIA NIH HHS F31 AG055326NIA NIH HHS K99 AG065200NIA NIH HHS R00 AG065200NIA NIH HHS R21 AG051047NIA NIH HHS R33 AG051047NIDDK NIH HHS T32 DK007647NIGMS NIH HHS R35 GM122589
6 · The paper itself

Abstract

Budding yeast, Saccharomyces cerevisiae, is an appealing model organism to study the organization and function of the actin cytoskeleton. With the advent of techniques to perform high-resolution, multidimensional analysis of the yeast cell, imaging of yeast has emerged as an important tool for research on the cytoskeleton. This chapter describes techniques and approaches for visualizing the actin cytoskeleton in fixed yeast cells with wide-field and super-resolution fluorescence microscopy.

Indexed as

Saccharomyces cerevisiaeActin CytoskeletonActinsCytoskeletonMicroscopy, FluorescenceActinsActinCytoskeletonFluorescent proteinsMicroscopySuper-resolution structured illumination microscopy (SR-SIM)Vital stainingYeast

Identifiers

PMID34542849
PMCPMC10131176

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.