ArticleBio-protocol2017
Comprehensive Methods for Leaf Geometric Morphometric Analyses.
Article in Bio-protocol, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed.
- Self-pollinated cannabis seeds lead to less variation in shape: a technological approach of potential commercial interest.BMC plant biology · 2025Article
- TPS5 and TOR signaling components are determinants ofFrontiers in plant science · 2025Article
- Differences in Leaf Morphology and Related Gene Expression between Diploid and Tetraploid Birch (International journal of molecular sciences · 2022Article
- Article
- Integrative Study of Genotypic and Phenotypic Diversity in the Eurasian Orchid GenusFrontiers in plant science · 2021Article
- Garryanalyzer: A morphometric workflow and open-source ImageJ plug-in for quantitative morphological analysis of Pacific NorthwestApplications in plant sciencesArticle
- From leaves to labels: Building modular machine learning networks for rapid herbarium specimen analysis with LeafMachine2.Applications in plant sciencesArticle
- Digital morphometrics: Application of MorphoLeaf in shape visualization and species delimitation, using Cucurbitaceae leaves as a model.Applications in plant sciencesArticle
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Leaf morphometrics are used frequently by several disciplines, including taxonomists, systematists, developmental biologists, morphologists, agronomists, and plant breeders to name just a few. Leaf shape is highly variable and can be used for identifying species or genotypes, developmental patterning within and among individuals, assessing plant health, and measuring environmental impacts on plant phenotype. Traditional leaf morphometrics requires hand tools and access to specimens, but modern efforts to digitize botanical collections make digital morphometrics a readily accessible and scientifically rigorous option. Here we provide detailed instructions for performing some of the most informative digital geometric morphometric analyses available: generalized Procrustes analysis, elliptical Fourier analysis, and shape features. This comprehensive procedure for leaf shape analysis is comprised of six main sections: A) scanning of material, B) acquiring landmarks, C) analysis of landmark data, D) isolating leaf outlines, E) analysis of leaf outlines, and F) shape features. This protocol provides a detailed reference for applying landmark and outline analysis to leaf shape as well as describing leaf shape features, thus empowering researchers to perform high throughput phenotyping for diverse applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.