Evidence map›Paper›PMID 34541249›Full record

ArticleBio-protocol2017

Measuring Cyanobacterial Metabolism in Biofilms with NanoSIMS Isotope Imaging and Scanning Electron Microscopy (SEM).

Rhona K Stuart, Xavier Mayali, Michael P Thelen, Jennifer Pett-Ridge, Peter K Weber

Abstract read
In one paragraph

Article in Bio-protocol, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Rhona K StuartPhysical and Life Sciences Division, Lawrence Livermore National Laboratory, Livermore, USA.
Xavier MayaliPhysical and Life Sciences Division, Lawrence Livermore National Laboratory, Livermore, USA.
Michael P ThelenPhysical and Life Sciences Division, Lawrence Livermore National Laboratory, Livermore, USA.
Jennifer Pett-RidgePhysical and Life Sciences Division, Lawrence Livermore National Laboratory, Livermore, USA.
Peter K WeberPhysical and Life Sciences Division, Lawrence Livermore National Laboratory, Livermore, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

To advance the understanding of microbial interactions, it is becoming increasingly important to resolve the individual metabolic contributions of microorganisms in complex communities. Organisms from biofilms can be especially difficult to separate, image and analyze, and methods to address these limitations are needed. High resolution imaging secondary ion mass spectrometry (NanoSIMS) generates single cell isotopic composition measurements, and can be used to quantify incorporation and exchange of an isotopically labeled substrate among individual organisms. Here, incorporation of cyanobacterial extracellular organic matter (EOM) by members of a cyanobacterial mixed species biofilm is used as a model to illustrate this method. Incorporation of stable isotope labeled (

Indexed as

Extracellular matrixExtracellular organic matterMicrobial ecologyNanoSIPStable isotopesSubstrate incorporation

Identifiers

PMID34541249
PMCPMC8410479

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.