ArticleBio-protocol2017
Ubiquitin Proteasome Activity Measurement in Total Plant Extracts.
Article in Bio-protocol, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 15 citations in OpenAlex.
- FaCPK19-FaTT1 axis decodes heat-induced CaThe New phytologist · 2026Article
- α-Asarone Maintains Protein Homeostasis Through SKN-1-Mediated Proteasome and Autophagy Pathways to Mitigate Aβ-Associated Toxicity inAntioxidants (Basel, Switzerland) · 2025Article
- Phloem sap from melon plants contains extracellular vesicles that carry active proteasomes which increase in response to aphid infestation.Journal of extracellular vesicles · 2024Article
- A natural allele of proteasome maturation factor improves rice resistance to multiple pathogens.Nature plants · 2023Article
- Multi-omic analysis shows REVEILLE clock genes are involved in carbohydrate metabolism and proteasome function.Plant physiology · 2022Article
- The Xanthomonas type-III effector XopS stabilizes CaWRKY40a to regulate defense responses and stomatal immunity in pepper (Capsicum annuum).The Plant cell · 2022Article
- Mild proteasomal stress improves photosynthetic performance in Arabidopsis chloroplasts.Nature communications · 2020Article
- Bacteria Exploit Autophagy for Proteasome Degradation and Enhanced Virulence in Plants.The Plant cell · 2018Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The fine-tuned balance of protein level, conformation and location within the cell is vital for the dynamic changes required for a cell to respond to a given stimulus. This requires the regulated turnover of damaged or short-lived proteins through the ubiquitin proteasome system (UPS). Thus, the protease activity of the proteasome is adjusted to meet the current demands of protein degradation via the UPS within the cell. We describe the adaptation of an intramolecular quenched fluorescence assay utilizing substrate-mimic peptides for the measurement of proteasome activity in total plant extracts. The peptide substrates contain donor-quencher pairs that flank the scissile bond. Following cleavage, the increase in dequenched donor emission of the product is subsequently measured over time and used to calculate the relative proteasome activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.