ArticleBio-protocol2017
TUNEL Assay to Assess Extent of DNA Fragmentation and Programmed Cell Death in Root Cells under Various Stress Conditions.
Article in Bio-protocol, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Programmed Cell Death in the Endosperm Is a Hallmark of Seed Germination inInternational journal of molecular sciences · 2026Article
- Article
- Wounding induces multilayered barrier formation in mature leaves via phytohormone signalling and ATML1-mediated epidermal specification.Nature plants · 2025Article
- ANAC044 orchestrates mitochondrial stress signaling to trigger iron-induced stem cell death in root meristems.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- How do rice seedlings of landrace Pokkali survive in saline fields after transplantation? Physiology, biochemistry, and photosynthesis.Photosynthesis research · 2021Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA damage is one of the common consequences of exposure to various stress conditions. Different methods have been developed to accurately assess DNA damage and fragmentation in cells and tissues exposed to different stress agents. However, owing to the presence of firm cellulosic cell wall and phenolics, plant cells and tissues are not easily amenable to be subjected to these assays. Here, we describe an optimized TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling) assay-based protocol to determine the extent of DNA fragmentation and programmed cell death in plant root cells subjected to various stress conditions. The method described here has the advantages of simplicity, reliability and reproducibility.
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