ArticleBio-protocol2021
Implementing Novel Designs in pET Expression Plasmids that Increase Protein Production.
Article in Bio-protocol, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Producing recombinant proteins in Vibrio natriegens.Microbial cell factories · 2024Article
- Computational construction of a glycoprotein multi-epitope subunit vaccine candidate for old and new South-African SARS-CoV-2 virus strains.Informatics in medicine unlocked · 2022Article
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Authors and funding
2 authors.
Funding
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Abstract
pET expression plasmids are widely used in the biotechnology, biopharmaceutical, and basic research sectors for the production of recombinant proteins. Typically, they are used off-the-shelf because they support high production titers; however, we have identified two design flaws in many pET plasmids that limit their production capacity. We used modern methods of DNA assembly and directed evolution to identify improved designs for these modules and demonstrated that these designs support higher protein production yields. Herein, we present two PCR protocols for implementing the designs and increasing protein production from existing pET expression plasmids. Graphic abstract: A simple workflow for implementing novel designs in pET expression plasmids.
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Registered trials
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