ArticlePhysical review. X
Pitching single-focus confocal data analysis one photon at a time with Bayesian nonparametrics.
Article in Physical review. X. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Article
- Perspective: An outlook on fluorescence tracking.The Journal of chemical physics · 2025Article
- Fluorescence correlation spectroscopy for particle sizing: A notorious challenge.Biophysical journal · 2025Review
- Quantitative analysis methods for free diffusion single-molecule FRET experiments.Current opinion in structural biology · 2025Review
- Physics-Inspired Single-Particle Tracking Accelerated with Parallelism.bioRxiv : the preprint server for biology · 2025Article
- Single-molecule reaction-diffusion.Biophysical journal · 2025Article
- Statistical analysis of fluorescence intensity transients with Bayesian methods.Science advances · 2025Article
- An accurate probabilistic step finder for time-series analysis.Biophysical journal · 2024Article
- Building Fluorescence Lifetime Maps Photon-by-Photon by Leveraging Spatial Correlations.ACS photonics · 2023Article
- Article
- An accurate probabilistic step finder for time-series analysis.bioRxiv : the preprint server for biology · 2023Article
- Single-Molecule Reaction-Diffusion.bioRxiv : the preprint server for biology · 2023Article
- Single-molecule FRET and molecular diffusion analysis characterize stable oligomers of amyloid-β 42 of extremely low population.PNAS nexus · 2023Article
- Analysis of photon trajectories from diffusing single molecules.The Journal of chemical physics · 2023Article
- Current capabilities and future perspectives of FCS: super-resolution microscopy, machine learning, and in vivo applications.Communications biology · 2023Review
- Monte Carlo samplers for efficient network inference.PLoS computational biology · 2023Article
- Single-photon smFRET: II. Application to continuous illumination.Biophysical reports · 2023Article
- Single-photon smFRET. I: Theory and conceptual basis.Biophysical reports · 2023Article
- Gene expression model inference from snapshot RNA data using Bayesian non-parametrics.Nature computational science · 2023Article
- Single-photon smFRET. III. Application to pulsed illumination.Biophysical reports · 2022Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Fluorescence time traces are used to report on dynamical properties of molecules. The basic unit of information in these traces is the arrival time of individual photons, which carry instantaneous information from the molecule, from which they are emitted, to the detector on timescales as fast as microseconds. Thus, it is theoretically possible to monitor molecular dynamics at such timescales from traces containing only a sufficient number of photon arrivals. In practice, however, traces are stochastic and in order to deduce dynamical information through traditional means-such as fluorescence correlation spectroscopy (FCS) and related techniques-they are collected and temporally autocorrelated over several minutes. So far, it has been impossible to analyze dynamical properties of molecules on timescales approaching data acquisition without collecting long traces under the strong assumption of stationarity of the process under observation or assumptions required for the analytic derivation of a correlation function. To avoid these assumptions, we would otherwise need to estimate the instantaneous number of molecules emitting photons and their positions within the confocal volume. As the number of molecules in a typical experiment is unknown, this problem demands that we abandon the conventional analysis paradigm. Here, we exploit Bayesian nonparametrics that allow us to obtain, in a principled fashion, estimates of the same quantities as FCS but from the direct analysis of traces of photon arrivals that are significantly smaller in size, or total duration, than those required by FCS.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.