Evidence map›Paper›PMID 34539243›Full record

ArticleMediators of inflammation2021

The Mutual Inhibition of FoxO1 and SREBP-1c Regulated the Progression of Hepatoblastoma by Regulating Fatty Acid Metabolism.

Yu Hu, Hongyan Zai, Wei Jiang, Zhenglin Ou, Yuanbing Yao, Qin Zhu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Mediators of inflammation, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.5field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Yu HuDepartment of General Surgery, Xiangya Hospital, Central South University, Changsha, 410008, China.
Hongyan ZaiDepartment of General Surgery, Xiangya Hospital, Central South University, Changsha, 410008, China.
Wei JiangDepartment of General Surgery, Xiangya Hospital, Central South University, Changsha, 410008, China.
Zhenglin OuDepartment of General Surgery, Xiangya Hospital, Central South University, Changsha, 410008, China.
Yuanbing YaoDepartment of General Surgery, Xiangya Hospital, Central South University, Changsha, 410008, China.
Qin ZhuDepartment of General Surgery, Xiangya Hospital, Central South University, Changsha, 410008, China.ORCID https://orcid.org/0000-0002-9864-5434
Central South University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHepatoblastoma (HB) is the most common liver malignancy in pediatrics, but the treatment for this disease is minimal. This study is aimed at exploring the effect of FoxO1 and SREBP-1c on HB and their mechanism.

methodsFoxO1, SREBP-1c, FASN, ACLY, ACC, and MAGL expressions in tissue samples were detected by RT-qPCR and WB. IHC was utilized to measure FASN content. Overexpression and knockdown of FoxO1 and sSREBP-1c were performed on Huh-6 cells. Cell proliferation, migration, and invasion were examined by CCK8, scratch, and transwell assay. ELISA was performed to test the ATP, FAO, NEFA, and Acetyl-CoA contents. ChIP was used to detect the interaction between SREBP-1c protein and the FoxO1 gene. In vivo tumorigenesis was conducted on mice. The morphology of tumor tissue sections was observed by HE staining.

resultsFoxO1 expression was downregulated in HB tissue, while the expressions of SREBP-1c, FASN, ACLY, ACC, and MAGL were upregulated. In Huh-6 cells and mouse tumor tissues, FoxO1 knockdown resulted in increased cell proliferation, migration, and invasion and active fatty acid metabolism. On the contrary, after the knockdown of SREBP-1c, cell proliferation, migration, and invasion were weakened, and fatty acid metabolism was significantly reduced. SREBP-1c interacted with the promoter of the FoxO1 gene. When FoxO1 was knocked down, the tumor tissue was more closely packed. After the knockdown of the SREBP-1c gene, the structure of tumor cells was deformed.

conclusionFoxO1 and SREBP-1c inhibited each other in HB, leading to the increase of intracellular fatty acid metabolism, and ultimately facilitated the development of HB.

Indexed as

Gene Expression RegulationAnimalsCell MovementCell ProliferationDisease ProgressionFatty AcidsFemaleForkhead Box Protein O1HepatoblastomaHumansImmunohistochemistryLipid MetabolismLiver NeoplasmsMiceMice, Inbred BALB CMice, NudeFatty AcidsForkhead Box Protein O1FOXO1 protein, humanFoxo1 protein, mouseSREBF1 protein, humanSrebf1 protein, mouseSterol Regulatory Element Binding Protein 1

Identifiers

PMID34539243
PMCPMC8443342
OpenAlexW3197963894

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.