Evidence map›Paper›PMID 34537856›Full record

ArticleInflammation research : official journal of the European Histamine Research Society ... [et al.]2021

Significance of glutathione peroxidase 4 and intracellular iron level in ovarian cancer cells-"utilization" of ferroptosis mechanism.

Dingxi Li, Mengli Zhang, Hongtu Chao

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Article in Inflammation research : official journal of the European Histamine Research Society ... [et al.], 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
4.5field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 42 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Dingxi LiDepartment of Gynecology, Affiliated Cancer Hospital of Zhengzhou University, 127 Dongming Road, Zhengzhou City, 450000, Henan Province, China.
Mengli ZhangDepartment of Gynecology, Affiliated Cancer Hospital of Zhengzhou University, 127 Dongming Road, Zhengzhou City, 450000, Henan Province, China.
Hongtu ChaoDepartment of Gynecology, Affiliated Cancer Hospital of Zhengzhou University, 127 Dongming Road, Zhengzhou City, 450000, Henan Province, China. hongtudazhan6661@163.com.ORCID http://orcid.org/0000-0002-4726-3006
Zhengzhou University · CN

Funding

the joint construction project of henan provincial medical science and technology tack key project in 2019 LHGJ20190657
6 · The paper itself

Abstract

OBJECTIVE AND

designOvarian cancer is the major cause of death in gynecologic diseases worldwide. Ferroptosis, a nonapoptotic form of cell death, is featured by accumulation of iron-based lipid peroxidation. The elevated iron level and malondialdehyde (MDA) in ovarian cancer cells suggest more vulnerable to ferroptosis, nevertheless, ferroptosis is not observed in ovarian cancer cells. Glutathione peroxidase 4 (GPX4) is a critical regulator of ferroptosis.

methodsWe determined whether GPX4 knockdown could induce ferroptosis to prevent cell proliferation in ovarian cancer. Human ovarian cancer cells and normal human ovarian epithelial cell line IOSE-80 were cultured and administrated with deferoxamine (DFO) or ferric ammonium citrate (FAC). GPX4 knockdown was established for investigating the functions of GPX4 in ovarian cancer cells and in tumor xenograft mice.

resultsA positively correlation was showed among the levels of GPX4, iron and cell proliferation. Chelation of intracellular iron by DFO disrupted intracellular iron level and was detrimental to ovarian cancer cell survival. FAC-induced elevation of intracellular iron inhibited proliferation, aggravated apoptosis, boosted inflammation and suppressed lipid peroxide reducibility in ovarian cancer cells. Knockdown of GPX4 had similar effects with FAC in ovarian cancer cells. Inhibition of GPX4 suppressed tumor growth, induced ferroptosis, accelerated cell apoptosis, reduced Fe

conclusionWe demonstrate the significance of GPX4 and intracellular iron level in ovarian cancer cells. Importantly, inhibition of GPX4 interferes with both intracellular iron homeostasis and lipid peroxide reducibility, inducing ferroptosis and exerting anti-cancer effect, which can be a potential effective strategy for ovarian cancer therapy.

Indexed as

FerroptosisAnimalsApoptosisCell LineCell ProliferationDeferoxamineFemaleFerric CompoundsHumansInterleukin-6IronMalondialdehydeMiceMice, Inbred BALB CMice, NudeOvarian NeoplasmsDeferoxamineferric ammonium citrateFerric CompoundsInterleukin-6IronMalondialdehydePhospholipid Hydroperoxide Glutathione PeroxidaseQuaternary Ammonium CompoundsTumor Necrosis Factor-alphaFerroptosisGlutathione peroxidase 4IronMalondialdehydeOvarian cancer

Identifiers

PMID34537856
OpenAlexW3199020838

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.