ArticleJournal of neuroscience methods2021
Dedifferentiation of radial glia-like cells is observed in in vitro explants of holothurian radial nerve cord.
Article in Journal of neuroscience methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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Who cites it
5 citing papers in PubMed, 7 citations in OpenAlex.
- Echinoderm Radial Glia-Like Cells Express Vertebrate Glial Markers.The Journal of comparative neurology · 2025Article
- Echinoderm radial glia in adult cell renewal, indeterminate growth, and regeneration.Frontiers in neural circuits · 2023Review
- The Stress Response of the Holothurian Central Nervous System: A Transcriptomic Analysis.International journal of molecular sciences · 2022Article
- Intestine Explants in Organ Culture: A Tool to Broaden the Regenerative Studies in Echinoderms.Journal of marine science and engineering · 2022Article
- Radial glia and radial glia-like cells: Their role in neurogenesis and regeneration.Frontiers in neuroscience · 2022Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundAmong animal phyla, some of the least studied nervous systems are those of the phylum Echinodermata. Part of the problem lies in that most of their nervous components are embedded in the body wall that has calcareous skeletal components. NEW
methodWe have developed a novel technique for the successful isolation of the radial nerve cords (RNCs) and an in vitro system where the isolated RNCs can be cultured and are amenable to experimental manipulation. Here we use this system to isolate the RNC of the sea cucumber Holothuria glaberrima as a way to extend our studies on its regeneration capabilities.
resultsThe RNCs can be isolated from the surrounding tissues by collagenase treatment. The explants obtained following enzymatic dissociation can be kept in culture for up to 2 weeks. Histological and immunohistochemical studies show that the explants maintain a stable number of cells with little proliferation or apoptosis throughout the culture incubation period. The main change observed in RNCs in vitro is a progressive dedifferentiation of radial glia-like cells. This dedifferentiation corresponds to the first step in the regeneration response to injury that has been described in vivo. COMPARISON WITH EXISTING
methodsThere are no existing methods to isolate and culture echinoderm radial nerve cord.
conclusionsThe described protocol provides a unique tool to obtain easily accessible RNC from holothurians to perform cellular, biochemical, and genomic experiments in the echinoderm nervous system without interference of adjacent tissues. The technique provides a unique opportunity to study the dedifferentiation response associated with the regeneration of the nervous system in echinoderms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.