ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2021
Multiscale Photoacoustic Tomography of a Genetically Encoded Near-Infrared FRET Biosensor.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Advanced methods of wearable ultrasound and photoacoustic imaging devices: A review.Photoacoustics · 2026Article
- Optoacoustic brain imaging: A systematic review and field mapping.Photoacoustics · 2026Article
- Characterization of Fluorophore-Quencher Pairs for Distance-Dependent Molecular Sensing in Photoacoustic Imaging.Chemical & biomedical imaging · 2026Article
- Photoacoustic Microscopy for Multiscale Biological System Visualization and Clinical Translation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Clinical translation of photoacoustic imaging using exogenous molecular contrast agents [Invited].Biomedical optics express · 2025Review
- Recent advancements in molecular photoacoustic tomography.JPhys photonics · 2025Review
- Deep-tissue high-sensitivity multimodal imaging and optogenetic manipulation enabled by biliverdin reductase knockout.Nature communications · 2025Article
- Enhanced dual-mode imaging: Superior photoacoustic and ultrasound endoscopy in live pigs using a transparent ultrasound transducer.Science advances · 2024Article
- Challenges and advances in two-dimensional photoacoustic computed tomography: a review.Journal of biomedical optics · 2024Review
- Next-Generation Genetically Encoded Fluorescent Biosensors Illuminate Cell Signaling and Metabolism.Annual review of biophysics · 2024Review
- Development of bright red-shifted miRFP704nano using structural analysis of miRFPnano proteins.Protein science : a publication of the Protein Society · 2023Article
- Multiscale photoacoustic tomography of neural activities with GCaMP calcium indicators.Journal of biomedical optics · 2022Article
- Looking deep inside tissue with photoacoustic molecular probes: a review.Journal of biomedical optics · 2022Review
- Transgenic mice encoding modern imaging probes: Properties and applications.Cell reports · 2022Review
- Optogenetic manipulation and photoacoustic imaging using a near-infrared transgenic mouse model.Nature communications · 2022Article
- Multiscale Photoacoustic Tomography of a Genetically Encoded Near-Infrared FRET Biosensor.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
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5 authors.
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Abstract
Photoacoustic tomography (PAT) with genetically encoded near-infrared probes enables visualization of specific cell populations in vivo at high resolution deeply in biological tissues. However, because of a lack of proper probes, PAT of cellular dynamics remains unexplored. Here, the authors report a near-infrared Forster resonance energy transfer (FRET) biosensor based on a miRFP670-iRFP720 pair of the near-infrared fluorescent proteins, which enables dynamic functional imaging of active biological processes in deep tissues. By photoacoustically detecting the changes in the optical absorption of the miRFP670 FRET-donor, they monitored cell apoptosis in deep tissue at high spatiotemporal resolution using PAT. Specifically, they detected apoptosis in single cells at a resolution of ≈3 µm in a mouse ear tumor, and in deep brain tumors (>3 mm beneath the scalp) of living mice at a spatial resolution of ≈150 µm with a 20 Hz frame rate. These results open the way for high-resolution photoacoustic imaging of dynamic biological processes in deep tissues using NIR biosensors and PAT.
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