ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2021
Choline acts during preimplantation development of the bovine embryo to program postnatal growth and alter muscle DNA methylation.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 40 citations in OpenAlex.
- Combined in ovo and post-hatch choline supplementation enhances growth and muscle development in goslings.Poultry science · 2026Article
- Metabolic and Lactation Effects of Rumen-Protected Choline Supplementation in Peripartum Dairy Cows and Its Effects on Calf Growth Until Weaning.Metabolites · 2026Article
- Oral administration of rumen-protected choline to ewes during the periconceptional period skews the sex ratio of lambs.Reproduction & fertility · 2026Article
- Effects of Rumen-Protected Methionine, Choline, and Betaine Supplementation on Ewes' Pregnancy and Reproductive Outcomes.Veterinary sciences · 2025Article
- Feeding rumen-protected choline during the periconceptional period programs postnatal phenotype of suckled beef calves.Journal of animal science and biotechnology · 2025Article
- Impact of paternal high energy diets on semen quality and embryo development in cattle.Reproduction & fertility · 2025Review
- Impact of dietary supplementation of beef cows with rumen-protected methionine during the periconceptional period on prenatal growth and performance to weaning.Journal of animal science · 2025Article
- A low concentration of choline chloride alters the developmental program of the bovine preimplantation embryo.Reproduction & fertility · 2024Article
- Systemic interindividual DNA methylation variants in cattle share major hallmarks with those in humans.Genome biology · 2024Article
- The Role of One-Carbon Metabolism and Methyl Donors in Medically Assisted Reproduction: A Narrative Review of the Literature.International journal of molecular sciences · 2024Review
- Pressing needs and recent advances to enhance production of embryos in vitro in cattle.Animal reproduction · 2024Article
- Molecular and cellular programs underlying the development of bovine pre-implantation embryos.Reproduction, fertility, and development · 2023Article
- Nutritional Strategies to Promote Bovine Oocyte Quality for In Vitro Embryo Production: Do They Really Work?Veterinary sciences · 2023Review
- Review: Some challenges and unrealized opportunities toward widespread use of the in vitro-produced embryo in cattle production.Animal : an international journal of animal bioscience · 2023Review
- Effect of the Supplementation Using an Herbal Mixture as a Choline Source during Early Gestation in Rambouillet Ewes.Animals : an open access journal from MDPI · 2023Article
- One-carbon metabolite supplementation to heifers for the first 14 d of the estrous cycle alters the plasma and hepatic one-carbon metabolite pool and methionine-folate cycle enzyme transcript abundance in a dose-dependent manner.Journal of animal science · 2023Article
- In utero choline exposure alters growth, metabolism, feed efficiency, and carcass characteristics of Holstein × Angus cattle from weaning to slaughter.Journal of animal science · 2023Article
- Differentially methylated regions identified in bovine embryos are not observed in adulthood.Animal reproduction · 2023Article
- Untargeted Metabolomics Reveals the Effect of Selective Breeding on the Quality of Chicken Meat.Metabolites · 2022Article
- Choline acts during preimplantation development of the bovine embryo to program postnatal growth and alter muscle DNA methylation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The preimplantation period of embryonic development can be a key window for programming of postnatal development because extensive epigenetic remodeling occurs during this time. It was hypothesized that modification of one-carbon metabolism of the bovine embryo by addition of the methyl-donor choline to culture medium would change postnatal phenotype through epigenetic modification. Embryos produced in vitro were cultured with 1.8 mM choline chloride or control medium. Blastocysts were transferred into females and pregnancy outcomes and postnatal phenotype of the resultant calves determined. Exposure of embryos to choline increased gestation length and calf birth weight. Calves derived from choline-treated embryos were also heavier at weaning and had increased ratio of body weight to hip height than control calves. Choline altered muscle DNA methylation of calves 4 months after birth. A total of 670 of the 8149 CpG examined were differentially methylated, with the predominant effect of choline being hypomethylation. Among the genes associated with differentially methylated CpG were ribosomal RNAs and genes in AMPK, mTOR, integrin, and BEX2 canonical pathways and cellular functions involved in growth and proliferation. Results demonstrate that provision of the methyl-donor choline to the preimplantation embryo can alter its developmental program to increase gestation length, birth weight, and weaning weight and cause postnatal changes in muscle DNA methylation including those associated with genes related to anabolic processes and cellular growth. The importance of the nutritional status of the embryo with respect to one-carbon metabolism for ensuring health and well-being after birth is emphasized by these observations.
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Registered trials
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