Evidence map›Paper›PMID 34533301›Full record

ArticleACS nano2021

Correlative Super-Resolution Optical and Atomic Force Microscopy Reveals Relationships Between Bacterial Cell Wall Architecture and Synthesis in

Raveen K G Tank, Victoria A Lund, Sandip Kumar, Robert D Turner, Lucia Lafage, Laia Pasquina Lemonche, Per A Bullough, Ashley Cadby, Simon J Foster, Jamie K Hobbs

Open access · hybridAbstract read
In one paragraph

Article in ACS nano, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
2.1field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 20 citations in OpenAlex.

  1. Case Study: Illuminating the Nanoscale World of Microbiology.Methods in molecular biology (Clifton, N.J.) · 2026
    Review
  2. Review
  3. Expanding the microbiologist toolboxMicrobiology spectrum · 2024
    Article
  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Raveen K G TankDepartment of Physics and Astronomy, University of Sheffield, Sheffield S3 7RH, United Kingdom.
Victoria A LundDepartment of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom.
Sandip KumarDepartment of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom.
Robert D TurnerDepartment of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom.
Lucia LafageDepartment of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom.ORCID 0000-0002-8105-5083
Laia Pasquina LemoncheDepartment of Physics and Astronomy, University of Sheffield, Sheffield S3 7RH, United Kingdom.
Per A BulloughDepartment of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom.
Ashley CadbyDepartment of Physics and Astronomy, University of Sheffield, Sheffield S3 7RH, United Kingdom.
Simon J FosterDepartment of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom.ORCID 0000-0001-7432-7805
Jamie K HobbsDepartment of Physics and Astronomy, University of Sheffield, Sheffield S3 7RH, United Kingdom.ORCID 0000-0002-5872-1404
University of Sheffield · GBUniversity of Oxford · GB

Funding

Biotechnology and Biological Sciences Research Council BB/I023518/1Medical Research Council MR/K015753/1Medical Research Council MR/N002679/1Medical Research Council MR/S014934/1Wellcome TrustWellcome Trust 212197/Z/19/Z
6 · The paper itself

Abstract

Understanding how bacteria grow and divide requires insight into both the molecular-level dynamics of ultrastructure and the chemistry of the constituent components. Atomic force microscopy (AFM) can provide near molecular resolution images of biological systems but typically provides limited chemical information. Conversely, while super-resolution optical microscopy allows localization of particular molecules and chemistries, information on the molecular context is difficult to obtain. Here, we combine these approaches into STORMForce (stochastic optical reconstruction with atomic force microscopy) and the complementary SIMForce (structured illumination with atomic force microscopy), to map the synthesis of the bacterial cell wall structural macromolecule, peptidoglycan, during growth and division in the rod-shaped bacterium

Indexed as

Bacillus subtilisCell WallMicroscopy, Atomic ForceMicroscopy, FluorescencePeptidoglycanPeptidoglycanatomic force microscopybacterial growthcorrelative microscopypeptidoglycanstochastic optical reconstruction microscopystructured illumination microscopysuper-resolution

Identifiers

PMID34533301
PMCPMC8552488
OpenAlexW3200321509

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.