Evidence map›Paper›PMID 34532823›Full record

ArticleBiotechnology letters2021

Rapid and cost-effective screening of CRISPR/Cas9-induced mutants by DNA-guided Argonaute nuclease.

Guohui Xiao, Xiangdong Fu, Juanjuan Zhang, Shuyan Liu, Zhaoqin Wang, Taosheng Ye, Guoliang Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology letters, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Guohui Xiao *National Clinical Research Center for Infectious Diseases, Southern University of Science and Technology, Shenzhen Third People's Hospital, Shenzhen, China. sswh12345@163.com.ORCID http://orcid.org/0000-0002-0298-6710
Xiangdong Fu *National Clinical Research Center for Infectious Diseases, Southern University of Science and Technology, Shenzhen Third People's Hospital, Shenzhen, China.
Juanjuan ZhangNational Clinical Research Center for Infectious Diseases, Southern University of Science and Technology, Shenzhen Third People's Hospital, Shenzhen, China.
Shuyan LiuNational Clinical Research Center for Infectious Diseases, Southern University of Science and Technology, Shenzhen Third People's Hospital, Shenzhen, China.
Zhaoqin WangNational Clinical Research Center for Infectious Diseases, Southern University of Science and Technology, Shenzhen Third People's Hospital, Shenzhen, China.
Taosheng YeNational Clinical Research Center for Infectious Diseases, Southern University of Science and Technology, Shenzhen Third People's Hospital, Shenzhen, China.
Guoliang ZhangNational Clinical Research Center for Infectious Diseases, Southern University of Science and Technology, Shenzhen Third People's Hospital, Shenzhen, China. szdsyy@aliyun.com.

Funding

National Basic Research Program of China (973 Program) 2020YFA0907200
6 · The paper itself

Abstract

objectiveWith the widespread application of CRISPR/Cas9 gene editing technology, new methods are needed to screen mutants quickly and effectively. Here, we aimed to develop a simple and cost-effective method to screen CRISPR/Cas9-induced mutants.

resultWe report a novel method to identify CRISPR/Cas9-induced mutants through a DNA-guided Argonaute nuclease derived from the archaeon Pyrococcus furiosus. We demonstrated that the Pyrococcus furiosus Argonaute (PfAgo)-based method could distinguish among biallelic mutants, monoallelic mutants and wild type (WT). Furthermore, this method was able to identify 1 bp indel mutations.

conclusionThe PfAgo-based method is simple to implement and can be applied to screen biallelic mutants and mosaic mutants generated by CRISPR-Cas9 or other kinds of gene editing tools.

Indexed as

Argonaute ProteinsGene EditingAnimalsArchaeal ProteinsCRISPR-Cas SystemsDNAINDEL MutationPyrococcus furiosusArchaeal ProteinsArgonaute ProteinsDNAArgonaute nucleaseBiallelic mutantsCRISPR/Cas9Monoallelic mutationsPyrococcus furiosus

Identifiers

PMID34532823
PMCPMC8445740

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.