ArticleOptica2020
High-contrast multifocus microscopy with a single camera and z-splitter prism.
Article in Optica, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The trial behind it
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Who cites it
18 citing papers in PubMed.
- Rapid axially scanned and de-scanned line-scan confocal microscopy with a tunable acoustic gradient index of refraction lens for high-speed volumetricNeurophotonics · 2025Article
- Multiplane 2.5D microscopy for high-throughput high-resolution tissue imaging.Journal of biomedical optics · 2025Article
- Volumetric imaging and computation to explore contractile function in zebrafish hearts.Cell reports methods · 2025Article
- Ultrafast optical imaging techniques for exploring rapid neuronal dynamics.Neurophotonics · 2025Review
- Multifocal microscopy for functional imaging of neural systems.Neurophotonics · 2024Article
- Tandem aberration correction optics (TACO) in wide-field structured illumination microscopy.Biomedical optics express · 2023Article
- Multiplane HiLo microscopy with speckle illumination and non-local means denoising.Journal of biomedical optics · 2023Article
- Article
- High-resolution multi-z confocal microscopy with a diffractive optical element.Biomedical optics express · 2023Article
- Design and Study of a Reflector-Separated Light Dispersion-Compensated 3D Microscopy System.Sensors (Basel, Switzerland) · 2023Article
- Mesoscale volumetric light-field (MesoLF) imaging of neuroactivity across cortical areas at 18 Hz.Nature methods · 2023Article
- The autism spectrum disorder risk geneFrontiers in neuroscience · 2023Article
- Article
- Fast volumetric imaging with line-scan confocal microscopy by electrically tunable lens at resonant frequency.Optics express · 2022Article
- KHz-rate volumetric voltage imaging of the whole Zebrafish heart.Biophysical reports · 2022Article
- Depth resolution in multifocus laser speckle contrast imaging.Optics letters · 2021Article
- High-speed multifocus phase imaging in thick tissue.Biomedical optics express · 2021Article
- Fast, multiplane line-scan confocal microscopy using axially distributed slits.Biomedical optics express · 2021Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Optical microscopy has been an indispensable tool for studying complex biological systems, but is often hampered by problems of speed and complexity when performing 3D volumetric imaging. Here, we present a multifocus imaging strategy based on the use of a simple z-splitter prism that can be assembled from off-the-shelf components. Our technique enables a widefield image stack to be distributed onto a single camera and recorded simultaneously. We exploit the volumetric nature of our image acquisition by further introducing a novel extended-volume 3D deconvolution strategy to suppress far-out-of-focus fluorescence background to significantly improve the contrast of our recorded images, conferring to our system a capacity for quasi-optical sectioning. By swapping in different z-splitter configurations, we can prioritize high speed or large 3D field-of-view imaging depending on the application of interest. Moreover, our system can be readily applied to a variety of imaging modalities in addition to fluorescence, such as phase-contrast and darkfield imaging. Because of its simplicity, versatility, and performance, we believe our system will be a useful tool for general biological or biomedical imaging applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.